Release Of The Cytosolic Tryparedoxin Peroxidase Into The Incubation Medium And A Different Profile Of Cytosolic And Mitochondrial Peroxiredoxin Expression In H2o2-treated Trypanosoma Cruzi Tissue Culture-derived Trypomastigotes.

dc.creatorGadelha, F R
dc.creatorGonçalves, C C
dc.creatorMattos, E C
dc.creatorAlves, M J M
dc.creatorPiñeyro, M D
dc.creatorRobello, C
dc.creatorPeloso, E F
dc.date2013-Mar
dc.date2015-11-27T13:32:40Z
dc.date2015-11-27T13:32:40Z
dc.date.accessioned2018-03-29T01:19:20Z
dc.date.available2018-03-29T01:19:20Z
dc.identifierExperimental Parasitology. v. 133, n. 3, p. 287-93, 2013-Mar.
dc.identifier1090-2449
dc.identifier10.1016/j.exppara.2012.12.007
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23266485
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/201031
dc.identifier23266485
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301264
dc.descriptionTrypanosoma cruzi antioxidant enzymes are among the factors that guarantee parasite survival and maintain infection, enabling T. cruzi to cope with oxidative stress. Herein, the expression of cytosolic (TcCPx) and mitochondrial (TcMPx) tryparedoxin peroxidases was evaluated in tissue culture-derived trypomastigotes upon incubation with different concentrations of H(2)O(2). TcCPx expression slightly increased (5.4%) in cells submitted to 10 μM H(2)O(2) treatment when compared to the control, but decreased when higher H(2)O(2) concentrations (20-50 μM) were employed. Under these conditions, TcMPx expression increased (∼53%) with 10 μM-treatment compared to the control, followed by a reduction that reached ∼46% of the control when using the highest concentration tested. Interestingly, in the supernatant of the incubations, TcCPx, but not TcMPx, was detected, and its levels increased concomitantly with its decreased expression in the intracellular compartment. Our data show that peroxiredoxins in the tissue culture-derived trypomastigote can be modulated under oxidative stress and are present in higher amounts when compared to the epimastigote stage of T. cruzi. Additionally, due to the different expression patterns observed upon H(2)O(2)-treatment, each peroxiredoxin may play a distinct role in protecting the parasite under oxidative stress conditions.
dc.description133
dc.description287-93
dc.languageeng
dc.relationExperimental Parasitology
dc.relationExp. Parasitol.
dc.rightsfechado
dc.rightsCopyright © 2012 Elsevier Inc. All rights reserved.
dc.sourcePubMed
dc.subjectBlotting, Western
dc.subjectCell Line
dc.subjectCytosol
dc.subjectHydrogen Peroxide
dc.subjectMitochondria
dc.subjectOxidative Stress
dc.subjectPeroxidases
dc.subjectPeroxiredoxins
dc.subjectProtozoan Proteins
dc.subjectTrypanosoma Cruzi
dc.titleRelease Of The Cytosolic Tryparedoxin Peroxidase Into The Incubation Medium And A Different Profile Of Cytosolic And Mitochondrial Peroxiredoxin Expression In H2o2-treated Trypanosoma Cruzi Tissue Culture-derived Trypomastigotes.
dc.typeArtículos de revistas


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