Exploring In Vivo Violacein Biosynthesis By Application Of Multivariate Curve Resolution On Fused Uv-vis Absorption, Fluorescence, And Liquid Chromatography-mass Spectrometry Data.

dc.creatorDantas, Clecio
dc.creatorTauler, Romà
dc.creatorFerreira, Márcia Miguel Castro
dc.date2013-Feb
dc.date2015-11-27T13:32:36Z
dc.date2015-11-27T13:32:36Z
dc.date.accessioned2018-03-29T01:19:12Z
dc.date.available2018-03-29T01:19:12Z
dc.identifierAnalytical And Bioanalytical Chemistry. v. 405, n. 4, p. 1293-302, 2013-Feb.
dc.identifier1618-2650
dc.identifier10.1007/s00216-012-6507-4
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23161064
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200999
dc.identifier23161064
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301232
dc.descriptionIn this work, the application of multivariate curve resolution-alternating least squares (MCR-ALS) is proposed for extracting information from multitechnique fused multivariate data (UV-VIS absorption, fluorescence, and liquid chromatography-mass spectrometry) gathered during the biosynthesis of violacein pigment. Experimental data sets were pretreated and arranged in a row-wise augmented data matrix before their chemometric investigation. Five different chemical components were resolved. Kinetic and spectral information about these components were obtained and their relationship with violacein biosynthesis was established. Three new chemical compounds with molar masses of 453, 465, and 479 u, until now not reported in the literature, were identified and proposed as intermediates in the biosynthesis of other indolocarbazoles. The precursor (tryptophan), one intermediate (deoxyviolacein), and the final product (violacein) of violacein biosynthesis were identified and characterized using the proposed approach. The chemometric procedure based on the MCR-ALS method has proved to be a powerful tool to investigate violacein biosynthesis and its application can be easily extended to the study of other bioprocesses.
dc.description405
dc.description1293-302
dc.languageeng
dc.relationAnalytical And Bioanalytical Chemistry
dc.relationAnal Bioanal Chem
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnti-bacterial Agents
dc.subjectChromatography, High Pressure Liquid
dc.subjectChromobacterium
dc.subjectIndoles
dc.subjectKinetics
dc.subjectLeast-squares Analysis
dc.subjectMass Spectrometry
dc.subjectSpectrometry, Fluorescence
dc.subjectSpectrophotometry, Ultraviolet
dc.titleExploring In Vivo Violacein Biosynthesis By Application Of Multivariate Curve Resolution On Fused Uv-vis Absorption, Fluorescence, And Liquid Chromatography-mass Spectrometry Data.
dc.typeArtículos de revistas


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