Effects Of Zoledronic Acid On Odontoblast-like Cells.

dc.creatorBasso, F G
dc.creatorTurrioni, A P S
dc.creatorHebling, J
dc.creatorde Souza Costa, C A
dc.date2013-May
dc.date2015-11-27T13:32:21Z
dc.date2015-11-27T13:32:21Z
dc.date.accessioned2018-03-29T01:18:51Z
dc.date.available2018-03-29T01:18:51Z
dc.identifierArchives Of Oral Biology. v. 58, n. 5, p. 467-73, 2013-May.
dc.identifier1879-1506
dc.identifier10.1016/j.archoralbio.2012.09.016
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23092609
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200907
dc.identifier23092609
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301140
dc.descriptionThe aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5×10(4)cells/cm(2)) were seeded for 48h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24h, ZOL (1 or 5μM) was added to the medium and maintained in contact with the cells for 24h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability - MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1μM ZOL caused a significant increase in Col-I expression. Although 5μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p<0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex.
dc.description58
dc.description467-73
dc.languageeng
dc.relationArchives Of Oral Biology
dc.relationArch. Oral Biol.
dc.rightsfechado
dc.rightsCopyright © 2012 Elsevier Ltd. All rights reserved.
dc.sourcePubMed
dc.subjectAlkaline Phosphatase
dc.subjectBone Density Conservation Agents
dc.subjectCell Survival
dc.subjectCells, Cultured
dc.subjectCollagen Type I
dc.subjectDiphosphonates
dc.subjectDose-response Relationship, Drug
dc.subjectExtracellular Matrix Proteins
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectHumans
dc.subjectImidazoles
dc.subjectOdontoblasts
dc.subjectPhosphoproteins
dc.subjectProtein Synthesis Inhibitors
dc.subjectSialoglycoproteins
dc.subjectSuccinate Dehydrogenase
dc.titleEffects Of Zoledronic Acid On Odontoblast-like Cells.
dc.typeArtículos de revistas


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