dc.creator | Basso, F G | |
dc.creator | Turrioni, A P S | |
dc.creator | Hebling, J | |
dc.creator | de Souza Costa, C A | |
dc.date | 2013-May | |
dc.date | 2015-11-27T13:32:21Z | |
dc.date | 2015-11-27T13:32:21Z | |
dc.date.accessioned | 2018-03-29T01:18:51Z | |
dc.date.available | 2018-03-29T01:18:51Z | |
dc.identifier | Archives Of Oral Biology. v. 58, n. 5, p. 467-73, 2013-May. | |
dc.identifier | 1879-1506 | |
dc.identifier | 10.1016/j.archoralbio.2012.09.016 | |
dc.identifier | http://www.ncbi.nlm.nih.gov/pubmed/23092609 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/200907 | |
dc.identifier | 23092609 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1301140 | |
dc.description | The aim of the study was to evaluate the effects of a highly potent bisphosphonate, zoledronic acid (ZOL), on cultured odontoblast-like cells MDPC-23. The cells (1.5×10(4)cells/cm(2)) were seeded for 48h in wells of 24-well dished. Then, the plain culture medium (DMEM) was replaced by fresh medium without fetal bovine serum. After 24h, ZOL (1 or 5μM) was added to the medium and maintained in contact with the cells for 24h. After this period, the succinic dehydrogenase (SDH) enzyme production (cell viability - MTT assay), total protein (TP) production, alkaline phosphatase (ALP) activity, and gene expression (qPCR) of collagen type I (Col-I) and ALP were evaluated. Cell morphology was assessed by SEM. Five μM ZOL caused a significant decrease in SDH production. Both ZOL concentrations caused a dose-dependent significant decrease in TP production and ALP activity. ZOL also produced discret morphological alterations in the MDPC-23 cells. Regarding gene expression, 1μM ZOL caused a significant increase in Col-I expression. Although 5μM ZOL did not affect Col-I expression, it caused a significant alteration in ALP expression (ANOVA and Tukey's test, p<0.05). ZOL presented a dose-dependent cytotoxic effect on the odontoblast-like cells, suggesting that under clinical conditions the release of this drug from dentin could cause damage to the pulpo-dentin complex. | |
dc.description | 58 | |
dc.description | 467-73 | |
dc.language | eng | |
dc.relation | Archives Of Oral Biology | |
dc.relation | Arch. Oral Biol. | |
dc.rights | fechado | |
dc.rights | Copyright © 2012 Elsevier Ltd. All rights reserved. | |
dc.source | PubMed | |
dc.subject | Alkaline Phosphatase | |
dc.subject | Bone Density Conservation Agents | |
dc.subject | Cell Survival | |
dc.subject | Cells, Cultured | |
dc.subject | Collagen Type I | |
dc.subject | Diphosphonates | |
dc.subject | Dose-response Relationship, Drug | |
dc.subject | Extracellular Matrix Proteins | |
dc.subject | Gene Expression Regulation, Enzymologic | |
dc.subject | Humans | |
dc.subject | Imidazoles | |
dc.subject | Odontoblasts | |
dc.subject | Phosphoproteins | |
dc.subject | Protein Synthesis Inhibitors | |
dc.subject | Sialoglycoproteins | |
dc.subject | Succinate Dehydrogenase | |
dc.title | Effects Of Zoledronic Acid On Odontoblast-like Cells. | |
dc.type | Artículos de revistas | |