Semiautomated Solid-phase Extraction Manifold With A Solvent-level Sensor.

dc.creatorOrlando, R M
dc.creatorRath, S
dc.creatorRohwedder, J J R
dc.date2013-Nov
dc.date2015-11-27T13:32:15Z
dc.date2015-11-27T13:32:15Z
dc.date.accessioned2018-03-29T01:18:40Z
dc.date.available2018-03-29T01:18:40Z
dc.identifierTalanta. v. 116, p. 39-44, 2013-Nov.
dc.identifier1873-3573
dc.identifier10.1016/j.talanta.2013.04.077
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24148370
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200856
dc.identifier24148370
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301089
dc.descriptionA semiautomated solid-phase extraction manifold for multiple extractions is presented. The manifold utilizes commercial solid-phase syringe cartridges and automatically introduces and elutes all the solvents during the extraction, reducing the typical workload and stress of the analyst. The manifold consists of a peristaltic pump with solenoid valves in a flow circuit that contains transmissive photomicrosensors. The photomicrosensors were used to control the solvent dispenser and the solvent level inside the cartridge. As solvent-level sensors, the photomicrosensors determined the exact time the solvent reached the top frit to avoid sorbent drying and accurately perform the solvent exchange. The repeatability of the manifold to introduce a particular volume of solvent into the cartridges was measured, and the precisions were between 0.05 and 2.89% (RSD). To evaluate the manifold, the amount of two fluoroquinolones in a fortified blank milk sample was determined. The results of the intra- and inter-day precision of multiple extractions from the fortified milk samples resulted in precisions better than 9.0% (RSD) and confirmed that the arrangement of the semiautomated manifold could adequately be used in solid-phase extraction with commercial cartridges.
dc.description116
dc.description39-44
dc.languageeng
dc.relationTalanta
dc.relationTalanta
dc.rightsfechado
dc.rightsCopyright © 2013 Elsevier B.V. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAnti-bacterial Agents
dc.subjectAutomation, Laboratory
dc.subjectCalibration
dc.subjectCiprofloxacin
dc.subjectFluoroquinolones
dc.subjectMilk
dc.subjectReproducibility Of Results
dc.subjectSensitivity And Specificity
dc.subjectSolid Phase Extraction
dc.subjectSolvents
dc.subjectFluoroquinolones
dc.subjectSemiautomated Solid-phase Extraction
dc.subjectSolvent-level Sensor
dc.titleSemiautomated Solid-phase Extraction Manifold With A Solvent-level Sensor.
dc.typeArtículos de revistas


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