Behavior Of Human Immunoglobulin G Adsorption Onto Immobilized Cu(ii) Affinity Hollow-fiber Membranes.

dc.creatorBorsoi-Ribeiro, Mariana
dc.creatorBresolin, Igor Tadeu Lazzarotto
dc.creatorVijayalakshmi, Mookambeswaran
dc.creatorBueno, Sônia Maria Alves
dc.date2013-Oct
dc.date2015-11-27T13:32:07Z
dc.date2015-11-27T13:32:07Z
dc.date.accessioned2018-03-29T01:18:26Z
dc.date.available2018-03-29T01:18:26Z
dc.identifierJournal Of Molecular Recognition : Jmr. v. 26, n. 10, p. 514-20, 2013-Oct.
dc.identifier1099-1352
dc.identifier10.1002/jmr.2296
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23996494
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200801
dc.identifier23996494
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301034
dc.descriptionIminodiacetic acid (IDA) and tris(2-aminoethyl)amine (TREN) chelating ligands were immobilized on poly(ethylene vinyl alcohol) (PEVA) hollow-fiber membranes after activation with epichlorohydrin or butanediol diglycidyl ether (bisoxirane). The affinity membranes complexed with Cu(II) were evaluated for adsorption of human immunoglobulin G (IgG). The effects of matrix activation and buffer system on adsorption of IgG were studied. Isotherms of batch IgG adsorption onto finely cut membranes showed that neither of the chelates, IDA-Cu(II) or TREN-Cu(II), had a Langmuirean behavior with negative cooperativity for IgG binding. A comparison of equilibrium and dynamic maximum capacities showed that the dynamic capacity for a mini-cartridge in a cross-flow filtration mode (52.5 and 298.4 mg g(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively) was somewhat higher than the equilibrium capacity (9.2 and 73.3 mg g(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively). When mini-cartridges were used, the dynamic adsorption capacity of IDA-Cu(II) was the same for both mini-cartridge and agarose gel.
dc.description26
dc.description514-20
dc.languageeng
dc.relationJournal Of Molecular Recognition : Jmr
dc.relationJ. Mol. Recognit.
dc.rightsfechado
dc.rightsCopyright © 2013 John Wiley & Sons, Ltd.
dc.sourcePubMed
dc.subjectAdsorption
dc.subjectCations, Divalent
dc.subjectChelating Agents
dc.subjectChromatography, Affinity
dc.subjectCopper
dc.subjectEpichlorohydrin
dc.subjectEpoxy Compounds
dc.subjectEthylenediamines
dc.subjectImino Acids
dc.subjectImmunoglobulin G
dc.subjectKinetics
dc.subjectMembranes, Artificial
dc.subjectPolyvinyls
dc.subjectProtein Binding
dc.subjectSolutions
dc.subjectThermodynamics
dc.subjectCu(ii)
dc.subjectImac
dc.subjectAdsorption
dc.subjectAffinity Membranes
dc.subjectHuman Igg
dc.subjectTris(2-aminoethyl)amine
dc.titleBehavior Of Human Immunoglobulin G Adsorption Onto Immobilized Cu(ii) Affinity Hollow-fiber Membranes.
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución