A Simple And Effective Set Of Pcr-based Molecular Markers For The Monitoring Of The Saccharomyces Cerevisiae Cell Population During Bioethanol Fermentation.

dc.creatorCarvalho-Netto, Osmar V
dc.creatorCarazzolle, Marcelo F
dc.creatorRodrigues, Aline
dc.creatorBragança, Welbe O
dc.creatorCosta, Gustavo G L
dc.creatorArgueso, Juan Lucas
dc.creatorPereira, Gonçalo A G
dc.date2013-Dec
dc.date2015-11-27T13:32:07Z
dc.date2015-11-27T13:32:07Z
dc.date.accessioned2018-03-29T01:18:26Z
dc.date.available2018-03-29T01:18:26Z
dc.identifierJournal Of Biotechnology. v. 168, n. 4, p. 701-9, 2013-Dec.
dc.identifier1873-4863
dc.identifier10.1016/j.jbiotec.2013.08.025
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23994268
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200800
dc.identifier23994268
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1301033
dc.descriptionOne of the defining features of the fermentation process used in the production of bioethanol from sugarcane feedstock is the dynamic nature of the yeast population. Minisatellite molecular markers are particularly useful for monitoring yeast communities because they produce polymorphic PCR products that typically display wide size variations. We compared the coding sequences derived from the genome of the sugarcane bioethanol strain JAY270/PE-2 to those of the reference Saccharomyces cerevisiae laboratory strain S288c, and searched for genes containing insertion or deletion polymorphisms larger than 24 bp. We then designed oligonucleotide primers flanking nine of these sites, and used them to amplify differentially sized PCR products. We analyzed the banding patterns in the most widely adopted sugarcane bioethanol strains and in several indigenous yeast contaminants, and found that our marker set had very good discriminatory power. Subsequently, these markers were used to successfully monitor the yeast cell populations in six sugarcane bioethanol distilleries. Additionally, we showed that most of the markers described here are also polymorphic among strains unrelated to bioethanol production, suggesting that they may be applied universally in S. cerevisiae. Because the relatively large polymorphisms are detectable in conventional agarose gels, our method is well suited to modestly equipped on-site laboratories at bioethanol distilleries, therefore providing both cost and time savings.
dc.description168
dc.description701-9
dc.languageeng
dc.relationJournal Of Biotechnology
dc.relationJ. Biotechnol.
dc.rightsfechado
dc.rightsCopyright © 2013 Elsevier B.V. All rights reserved.
dc.sourcePubMed
dc.subjectBiofuels
dc.subjectEthanol
dc.subjectFermentation
dc.subjectGenetic Markers
dc.subjectIndustrial Microbiology
dc.subjectPolymerase Chain Reaction
dc.subjectPolymorphism, Genetic
dc.subjectSaccharomyces Cerevisiae
dc.subjectSaccharum
dc.subjectBioethanol
dc.subjectGenotyping
dc.subjectPcr
dc.subjectSaccharomyces Cerevisiae
dc.titleA Simple And Effective Set Of Pcr-based Molecular Markers For The Monitoring Of The Saccharomyces Cerevisiae Cell Population During Bioethanol Fermentation.
dc.typeArtículos de revistas


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