Involvement Of Nitrite In The Nitrate-mediated Modulation Of Fermentative Metabolism And Nitric Oxide Production Of Soybean Roots During Hypoxia.

dc.creatorOliveira, Halley C
dc.creatorSalgado, Ione
dc.creatorSodek, Ladaslav
dc.date2013-Jan
dc.date2015-11-27T13:32:02Z
dc.date2015-11-27T13:32:02Z
dc.date.accessioned2018-03-29T01:18:18Z
dc.date.available2018-03-29T01:18:18Z
dc.identifierPlanta. v. 237, n. 1, p. 255-64, 2013-Jan.
dc.identifier1432-2048
dc.identifier10.1007/s00425-012-1773-0
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23011570
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200764
dc.identifier23011570
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300997
dc.descriptionIt is widely accepted that nitrate but not ammonium improves tolerance of plants to hypoxic stress, although the mechanisms related to this beneficial effect are not well understood. Recently, nitrite derived from nitrate reduction has emerged as the major substrate for the synthesis of nitric oxide (NO), an important signaling molecule in plants. Here, we analyzed the effect of different nitrogen sources (nitrate, nitrite and ammonium) on the metabolic response and NO production of soybean roots under hypoxia. Organic acid analysis showed that root segments isolated from nitrate-cultivated plants presented a lower accumulation of lactate and succinate in response to oxygen deficiency in relation to those from ammonium-cultivated plants. The more pronounced lactate accumulation by root segments of ammonium-grown plants was followed by a higher ethanol release in the medium, evidencing a more intense fermentation under oxygen deficiency than those from nitrate-grown plants. As expected, root segments from nitrate-cultivated plants produced higher amounts of nitrite and NO during hypoxia compared to ammonium cultivation. Exogenous nitrite supplied during hypoxia reduced both ethanol and lactate production and stimulated cyanide-sensitive NO emission by root segments from ammonium-cultivated plants, independent of nitrate. On the other hand, treatments with a NO donor or a NO scavenger did not affect the intensity of fermentation of soybean roots. Overall, these results indicate that nitrite participates in the nitrate-mediated modulation of the fermentative metabolism of soybean roots during oxygen deficiency. The involvement of mitochondrial reduction of nitrite to NO in this mechanism is discussed.
dc.description237
dc.description255-64
dc.languageeng
dc.relationPlanta
dc.relationPlanta
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectBenzoates
dc.subjectCarboxylic Acids
dc.subjectFermentation
dc.subjectGas Chromatography-mass Spectrometry
dc.subjectImidazoles
dc.subjectLactates
dc.subjectNitrates
dc.subjectNitric Oxide
dc.subjectNitric Oxide Donors
dc.subjectNitrites
dc.subjectOxygen
dc.subjectPlant Roots
dc.subjectQuaternary Ammonium Compounds
dc.subjectSoybeans
dc.subjectSuccinates
dc.subjectTime Factors
dc.subjectTriazenes
dc.titleInvolvement Of Nitrite In The Nitrate-mediated Modulation Of Fermentative Metabolism And Nitric Oxide Production Of Soybean Roots During Hypoxia.
dc.typeArtículos de revistas


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