Inhibition Of Human Platelet Aggregation By Eosinophils.

dc.creatorMaziero, Aline Mendes
dc.creatorLorenzetti, Raquel
dc.creatorDonato, José Luiz
dc.creatorLilla, Sergio
dc.creatorDe Nucci, Gilberto
dc.date2013-Sep
dc.date2015-11-27T13:31:58Z
dc.date2015-11-27T13:31:58Z
dc.date.accessioned2018-03-29T01:18:11Z
dc.date.available2018-03-29T01:18:11Z
dc.identifierLife Sciences. v. 93, n. 9-11, p. 416-22, 2013-Sep.
dc.identifier1879-0631
dc.identifier10.1016/j.lfs.2013.07.012
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23871985
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200738
dc.identifier23871985
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300971
dc.descriptionThe relationship between the activity of eosinophils and platelets has been observed in recent decades by many scientists. These observations include increased numbers of eosinophils associated with platelet disorders, including changes in the coagulation cascade and platelet aggregation. Based on these observations, the interaction between eosinophils and platelets in platelet aggregation was analyze. Human platelets were incubated with eosinophil cytosolic fraction, promyelocytic human HL-60 clone 15 cell lineage, and eosinophil cationic protein (ECP). Platelet rich plasma (PRP) aggregation was induced by adenosine diphosphate, platelet activating factor, arachidonic acid, and collagen, and washed platelets (WP) were activated by thrombin. Aggregation induced by all agonists was dose dependently inhibited by eosinophil cytosolic fraction. This inhibition was only partially reversed by previous incubation of the eosinophils with l-Nitro-Arginine-Methyl-Ester (l-NAME). Previous incubation with indomethacin did not prevent the cytosolic fraction induced inhibition. The separation of eosinophil cytosolic fraction by gel filtration on Sephadex G-75 showed that the inhibitory activity was concentrated in the lower molecular weight fraction. HL-60 clone 15 cells differentiated into eosinophils for 5 and 7 day were able to inhibit platelet aggregation. The ECP protein inhibited the platelet aggregation on PRP and WP. This inhibition was more evident in WP, and the citotoxicity MTT assay proved the viability of tested platelets, showing that the observed inhibition by the ECP protein does not occur simply by cell death. Our results indicate that eosinophils play a fundamental role in platelet aggregation inhibition.
dc.description93
dc.description416-22
dc.languageeng
dc.relationLife Sciences
dc.relationLife Sci.
dc.rightsfechado
dc.rights© 2013.
dc.sourcePubMed
dc.subjectAdenosine Diphosphate
dc.subjectAnimals
dc.subjectArachidonic Acid
dc.subjectBlood Platelets
dc.subjectChromatography, Gel
dc.subjectCollagen
dc.subjectCytosol
dc.subjectDextrans
dc.subjectEosinophils
dc.subjectHl-60 Cells
dc.subjectHumans
dc.subjectIndomethacin
dc.subjectMale
dc.subjectMolecular Weight
dc.subjectNg-nitroarginine Methyl Ester
dc.subjectPlatelet Activating Factor
dc.subjectPlatelet Aggregation
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectThrombin
dc.subjectTime Factors
dc.subjectEosinophil Cationic Protein
dc.subjectEosinophil Cytosolic Fraction
dc.subjectEosinophil Proteins
dc.subjectPlatelet Aggregation Inhibition
dc.titleInhibition Of Human Platelet Aggregation By Eosinophils.
dc.typeArtículos de revistas


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