Characterization Of A Refinement Of The Pylorus Ligation" Model Of Rat Gastric Ulceration Resulting In "no Pain" And A More Specific Pharmacological Response."

dc.creatorMonteiro, Karin M
dc.creatorSpindola, Humberto M
dc.creatorPossenti, Ana
dc.creatorTinti, Sirlene V
dc.creatorRuiz, Ana L T G
dc.creatorLongato, Giovanna B
dc.creatorFiorito, Giovanna F
dc.creatorMarchetti, Gabriela M
dc.creatorShiozawa, Larissa
dc.creatorPiloni, Bárbara U
dc.creatorde Oliveira, Ana C
dc.creatorMiyagawa, Livia M
dc.creatorCarvalho, João E
dc.date
dc.date2015-11-27T13:31:57Z
dc.date2015-11-27T13:31:57Z
dc.date.accessioned2018-03-29T01:18:10Z
dc.date.available2018-03-29T01:18:10Z
dc.identifierJournal Of Pharmacological And Toxicological Methods. v. 67, n. 2, p. 121-8
dc.identifier1873-488X
dc.identifier10.1016/j.vascn.2012.09.002
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23000280
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200731
dc.identifier23000280
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300964
dc.descriptionThe pharmacological assessment of the factors for gastric protection of a test substance should involve experimental models that can determine the involvement of cytoprotective factors, as well as their influence on the secretion of hydrochloric acid. The original protocol of pylorus ligation in rats proposed by Shay et al. in 1945, still in use today, provides a latency time of 240 min without considering the effect of postoperative pain in the mechanisms of peptic ulcer. This paper proposes a modification of this experimental protocol by eliminating the pain throughout the postoperative period, as a refinement of the test with consequent improvement of the pharmacological response. Adult male Wistar/Uni rats underwent surgical ligation of the pylorus and were kept anesthetized throughout the experimental period (4h) in contrast to the other experimental groups that followed the original protocol proposed by Shay et al., 1945. We were able to determine effective doses for a positive control, as well as of a variety of secretagogues in the new experimental protocol proposed. The suppression of post-surgical pain, through the use of anesthesia throughout the experimental period, brought several benefits for the study of gastric acid secretion, rendering a more homogeneous pharmacologic response in non-inbred animals, thus being an effective experimental procedure.
dc.description67
dc.description121-8
dc.languageeng
dc.relationJournal Of Pharmacological And Toxicological Methods
dc.relationJ Pharmacol Toxicol Methods
dc.rightsfechado
dc.rightsCopyright © 2012 Elsevier Inc. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBethanechol
dc.subjectDisease Models, Animal
dc.subjectDose-response Relationship, Drug
dc.subjectGastric Acid
dc.subjectHistamine
dc.subjectLigation
dc.subjectMale
dc.subjectPain, Postoperative
dc.subjectPentagastrin
dc.subjectPylorus
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSensitivity And Specificity
dc.subjectStomach
dc.subjectStomach Ulcer
dc.subjectStress, Physiological
dc.titleCharacterization Of A Refinement Of The Pylorus Ligation" Model Of Rat Gastric Ulceration Resulting In "no Pain" And A More Specific Pharmacological Response."
dc.typeArtículos de revistas


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