Influence Of Protein Corona On The Transport Of Molecules Into Cells By Mesoporous Silica Nanoparticles.

dc.creatorPaula, Amauri J
dc.creatorAraujo Júnior, Roberto T
dc.creatorMartinez, Diego Stéfani T
dc.creatorParedes-Gamero, Edgar J
dc.creatorNader, Helena B
dc.creatorDurán, Nelson
dc.creatorJusto, Giselle Z
dc.creatorAlves, Oswaldo Luiz
dc.date2013-Sep
dc.date2015-11-27T13:31:56Z
dc.date2015-11-27T13:31:56Z
dc.date.accessioned2018-03-29T01:18:07Z
dc.date.available2018-03-29T01:18:07Z
dc.identifierAcs Applied Materials & Interfaces. v. 5, n. 17, p. 8387-93, 2013-Sep.
dc.identifier1944-8252
dc.identifier10.1021/am4014693
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23841723
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200721
dc.identifier23841723
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300954
dc.descriptionAlthough there are several studies reporting the promising biological efficiency of mesoporous silica nanoparticles (loaded with antitumoral drugs) against cancer cells and tumors, there are no reports on the influence of the bio-nano interface interactions on the molecular diffusion process occurring along their pores. In this context, we show here that the protein coating formed on multifunctionalized colloidal mesoporous silica nanoparticles (MSNs) dispersed in a cell culture medium decreases the release of camptothecin (CPT, a hydrophobic antitumoral drug) from the pores of MSNs. This effect is related to the adsorption of biomolecules on the nanoparticle surface, which partially blocks the pores. Parallely, the hydrophobic functionalization inside the pores can offer suitable sites for the adsorption of other molecules present in the cell culture medium depending on the hydrophobicity, size, and conformation aspects of these molecules and adsorption sites of MSNs. Thus, the molecular cargo loaded in the pores (i.e. CPT) can be replaced by specific molecules present in the dispersion medium. As a consequence, we show that a non-permeable cellular staining molecule such as SYTOX green can be incorporated in MSNs through this mechanism and internalized by cells in an artificial fashion. By extrapolating this phenomenon for applications in vivo, one has to consider now the possible manifestation of unpredicted biological effects from the use of porous silica nanoparticles and others with similar structure due to these internalization aspects.
dc.description5
dc.description8387-93
dc.languageeng
dc.relationAcs Applied Materials & Interfaces
dc.relationACS Appl Mater Interfaces
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdsorption
dc.subjectAnimals
dc.subjectAntineoplastic Agents, Phytogenic
dc.subjectCamptothecin
dc.subjectCattle
dc.subjectCell Survival
dc.subjectDrug Carriers
dc.subjectHct116 Cells
dc.subjectHumans
dc.subjectHydrophobic And Hydrophilic Interactions
dc.subjectMicroscopy, Confocal
dc.subjectNanoparticles
dc.subjectPorosity
dc.subjectSerum Albumin, Bovine
dc.subjectSilicon Dioxide
dc.titleInfluence Of Protein Corona On The Transport Of Molecules Into Cells By Mesoporous Silica Nanoparticles.
dc.typeArtículos de revistas


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