| dc.creator | Pessôa, Gustavo de Souza | |
| dc.creator | Pilau, Eduardo Jorge | |
| dc.creator | Gozzo, Fábio Cesar | |
| dc.creator | Arruda, Marco Aurélio Zezzi | |
| dc.date | 2013-Sep | |
| dc.date | 2015-11-27T13:31:47Z | |
| dc.date | 2015-11-27T13:31:47Z | |
| dc.date.accessioned | 2018-03-29T01:17:52Z | |
| dc.date.available | 2018-03-29T01:17:52Z | |
| dc.identifier | Analytical And Bioanalytical Chemistry. v. 405, n. 24, p. 7653-60, 2013-Sep. | |
| dc.identifier | 1618-2650 | |
| dc.identifier | 10.1007/s00216-013-7064-1 | |
| dc.identifier | http://www.ncbi.nlm.nih.gov/pubmed/23722891 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/200656 | |
| dc.identifier | 23722891 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1300889 | |
| dc.description | In the present work, traveling wave ion mobility spectrometry-mass spectrometry (TWIMS-MS) was applied to speciation analysis of metalloproteins. The influence of pH on complexation conditions between some metals and bovine carbonic anhydrase was evaluated from pH 6 to 9, as well as the time involved in their complexation (0-24 h). Employing TWIMS-MS, two conformational states of bovine carbonic anhydrase were observed with charge states of +12 and +11; these configurations being evaluated in terms of the folded state of the apo form and this protein (at charge state +11) being linked to barium, lead, copper, and zinc in their divalent forms. Metalloprotein speciation analysis was carried out for copper (Cu(+) and Cu(2+)), lead (Pb(2+) and Pb(4+)), and selenium (Se(4+) and Se(6+)) species complexed with bovine carbonic anhydrase. Mobilities of all complexed species were compared, also considering the apo form of this protein. | |
| dc.description | 405 | |
| dc.description | 7653-60 | |
| dc.language | eng | |
| dc.relation | Analytical And Bioanalytical Chemistry | |
| dc.relation | Anal Bioanal Chem | |
| dc.rights | fechado | |
| dc.rights | | |
| dc.source | PubMed | |
| dc.subject | Carbonic Anhydrases | |
| dc.subject | Hydrogen-ion Concentration | |
| dc.subject | Ion Transport | |
| dc.subject | Metalloids | |
| dc.subject | Metals | |
| dc.subject | Protein Binding | |
| dc.subject | Spectrometry, Mass, Electrospray Ionization | |
| dc.title | Ion Mobility Spectrometry Focusing On Speciation Analysis Of Metals/metalloids Bound To Carbonic Anhydrase. | |
| dc.type | Artículos de revistas | |
