Standardization Of Histological Procedures For The Detection Of Toxic Substances By Immunohistochemistry In Dipteran Larvae Of Forensic Importance.

dc.creatorSouza, Carina M
dc.creatorLima, Carolina G P
dc.creatorAlves, Marcos J
dc.creatorArrais-Silva, Wagner W
dc.creatorGiorgio, Selma
dc.creatorLinhares, Arício X
dc.creatorThyssen, Patricia J
dc.date2013-Jul
dc.date2015-11-27T13:31:44Z
dc.date2015-11-27T13:31:44Z
dc.date.accessioned2018-03-29T01:17:48Z
dc.date.available2018-03-29T01:17:48Z
dc.identifierJournal Of Forensic Sciences. v. 58, n. 4, p. 1015-21, 2013-Jul.
dc.identifier1556-4029
dc.identifier10.1111/1556-4029.12140
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23682832
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200635
dc.identifier23682832
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300868
dc.descriptionImmunohistochemistry (IHC) technique is an alternative toxicological analysis to detect drugs in insects of forensic importance, but it requires thorough histological procedures. In this study, we tested different fixatives--phosphate-buffered paraformaldehyde 4% (PP), Carnoy's fluid (CF), Kahle's solution (KS), ethanol in different concentrations, and ethanol associated to PP and CF, time of fixation and histological processes for dipteran larvae's tissue, aiming to develop a sample preparation protocol for IHC application. A suitable fixation was achieved using PP for 12 and 24 h, CF for 3 h, 70% ethanol for 19 days, and 70% ethanol/CF for 2 h/3 h. Postfixation using negative pressure, two immersions in xylene for 30 min each, and one in xylene plus paraffin for 45 min increased tissue preservation. An immunohistochemical test for cocaine detection was performed using monoclonal benzoylecgonine antibody from mouse, peroxidase-conjugated anti-mouse IgG and visualized by 3,3'-diaminobenzidine method showed these histological procedures didn't compromise antigenicity.
dc.description58
dc.description1015-21
dc.languageeng
dc.relationJournal Of Forensic Sciences
dc.relationJ. Forensic Sci.
dc.rightsfechado
dc.rights© 2013 American Academy of Forensic Sciences.
dc.sourcePubMed
dc.subjectAcetic Acid
dc.subjectAnimals
dc.subjectChloroform
dc.subjectCocaine
dc.subjectDiptera
dc.subjectEntomology
dc.subjectEthanol
dc.subjectFeeding Behavior
dc.subjectFixatives
dc.subjectForensic Pathology
dc.subjectForensic Toxicology
dc.subjectFormaldehyde
dc.subjectImmunohistochemistry
dc.subjectLarva
dc.subjectNarcotics
dc.subjectParaffin
dc.subjectPolymers
dc.subjectSpecimen Handling
dc.subjectTime Factors
dc.subjectXylenes
dc.subjectEntomotoxicology
dc.subjectFixatives
dc.subjectForensic Entomology
dc.subjectForensic Science
dc.subjectHistological Technique
dc.subjectImmunohistochemical Analyses
dc.titleStandardization Of Histological Procedures For The Detection Of Toxic Substances By Immunohistochemistry In Dipteran Larvae Of Forensic Importance.
dc.typeArtículos de revistas


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