Modulatory Effects Of Yerba Maté (ilex Paraguariensis) On The Pi3k-akt Signaling Pathway.

dc.creatorArçari, Demétrius Paiva
dc.creatorSantos, Juliana Carvalho
dc.creatorGambero, Alessandra
dc.creatorFerraz, Lucio Fábio Caldas
dc.creatorRibeiro, Marcelo Lima
dc.date2013-Oct
dc.date2015-11-27T13:31:44Z
dc.date2015-11-27T13:31:44Z
dc.date.accessioned2018-03-29T01:17:47Z
dc.date.available2018-03-29T01:17:47Z
dc.identifierMolecular Nutrition & Food Research. v. 57, n. 10, p. 1882-5, 2013-Oct.
dc.identifier1613-4133
dc.identifier10.1002/mnfr.201200834
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23670797
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200633
dc.identifier23670797
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300866
dc.descriptionThe aim of this study was to evaluate the effects of yerba maté (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 g/kg. After treatment, glucose blood level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba maté extract significantly decreased the final body weight, glucose blood levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba maté treatment, the levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba maté on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.
dc.description57
dc.description1882-5
dc.languageeng
dc.relationMolecular Nutrition & Food Research
dc.relationMol Nutr Food Res
dc.rightsfechado
dc.rights© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBlood Glucose
dc.subjectBody Weight
dc.subjectDiet, High-fat
dc.subjectDown-regulation
dc.subjectForkhead Transcription Factors
dc.subjectGlucose-6-phosphatase
dc.subjectIlex Paraguariensis
dc.subjectInsulin
dc.subjectInsulin Receptor Substrate Proteins
dc.subjectInsulin Resistance
dc.subjectMale
dc.subjectMice
dc.subjectPhosphatidylinositol 3-kinase
dc.subjectProtein-serine-threonine Kinases
dc.subjectProto-oncogene Proteins C-akt
dc.subjectRna, Messenger
dc.subjectSignal Transduction
dc.subjectUp-regulation
dc.subjectInsulin Resistance
dc.subjectObesity
dc.subjectPi3k-akt
dc.subjectYerba Maté
dc.titleModulatory Effects Of Yerba Maté (ilex Paraguariensis) On The Pi3k-akt Signaling Pathway.
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución