Chondrogenesis From Umbilical Cord Blood Cells Stimulated With Bmp-2 And Bmp-6.

dc.creatorde Mara, Cristiane Sampaio
dc.creatorDuarte, A S S
dc.creatorSartori-Cintra, A R
dc.creatorLuzo, A C M
dc.creatorSaad, S T O
dc.creatorCoimbra, I B
dc.date2013-Jan
dc.date2015-11-27T13:31:09Z
dc.date2015-11-27T13:31:09Z
dc.date.accessioned2018-03-29T01:16:56Z
dc.date.available2018-03-29T01:16:56Z
dc.identifierRheumatology International. v. 33, n. 1, p. 121-8, 2013-Jan.
dc.identifier1437-160X
dc.identifier10.1007/s00296-011-2328-6
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/22238025
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200416
dc.identifier22238025
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300649
dc.descriptionUmbilical cord blood contains undifferentiated mesenchymal stem cells (MSCs) with chondrogenic potential that may be used for the repair of joint damage. The role of growth factors during the process of chondrogenesis is still not entirely understood. The objective of this study was to evaluate the formation of chondrocytes, cartilaginous matrix and type II collagen from human umbilical cord blood stem cells exposed to two different growth factors, BMP-6 and BMP-2, while being cultured as a micromass or a monolayer. Umbilical cord blood was obtained from full-term deliveries, and then, mononuclear cells were separated and cultured for expansion. Afterward, these cells were evaluated by flow cytometry using antibodies specific for MSCs and induced to chondrogenic differentiation in micromass and monolayer cultures supplemented with BMP-2 and BMP-6. Cellular phenotype was evaluated after 7, 14 and 21 days by RT-PCR and Western blot analysis to identify the type II collagen and aggrecan. The expanded cells displayed surface antigens characteristic of mesenchymal progenitor cells and were negative for hematopoietic differentiation antigens. Type II collagen and aggrecan mRNAs were expressed from day 14 in cells stimulated with BMP-2 or BMP-6. Type II collagen was demonstrated by Western blotting in both groups, and the greatest expression was observed 21 days after the cells were stimulated with BMP-2 cultured in micromass. BMP-2 in micromass culture was more efficient to induce the chondrogenesis.
dc.description33
dc.description121-8
dc.languageeng
dc.relationRheumatology International
dc.relationRheumatol. Int.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAggrecans
dc.subjectBone Morphogenetic Protein 2
dc.subjectBone Morphogenetic Protein 6
dc.subjectCell Differentiation
dc.subjectCells, Cultured
dc.subjectChondrocytes
dc.subjectChondrogenesis
dc.subjectCollagen Type Ii
dc.subjectExtracellular Matrix Proteins
dc.subjectFetal Blood
dc.subjectGene Expression
dc.subjectHumans
dc.subjectMesenchymal Stromal Cells
dc.titleChondrogenesis From Umbilical Cord Blood Cells Stimulated With Bmp-2 And Bmp-6.
dc.typeArtículos de revistas


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