Contribution Of At-, Gc-, And Methylated Cytidine-rich Dna To Chromatin Composition In Malpighian Tubule Cell Nuclei Of Panstrongylus Megistus (hemiptera, Reduviidae).

dc.creatorAlvarenga, Elenice M
dc.creatorMondin, Mateus
dc.creatorRodrigues, Vera L C C
dc.creatorAndrade, Larissa M
dc.creatorVidal, Benedicto de Campos
dc.creatorMello, Maria Luiza S
dc.date2012-Nov
dc.date2015-11-27T13:29:17Z
dc.date2015-11-27T13:29:17Z
dc.date.accessioned2018-03-29T01:16:54Z
dc.date.available2018-03-29T01:16:54Z
dc.identifierActa Histochemica. v. 114, n. 7, p. 665-72, 2012-Nov.
dc.identifier1618-0372
dc.identifier10.1016/j.acthis.2011.12.001
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/22197484
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200408
dc.identifier22197484
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300641
dc.descriptionThe Malpighian tubule cell nuclei of male Panstrongylus megistus, a vector of Chagas disease, contain one chromocenter, which is composed solely of the Y chromosome. Considering that different chromosomes contribute to the composition of chromocenters in different triatomini species, the aim of this study was to determine the contribution of AT-, GC-, and methylated cytidine-rich DNA in the chromocenter as well as in euchromatin of Malpighian tubule cell nuclei of P. megistus in comparison with published data for Triatoma infestans. Staining with 4',6-diamidino-2-phenylindole/actinomycin D and chromomycin A(3)/distamycin, immunodetection of 5-methylcytidine and AgNOR test were used. The results revealed AT-rich/GC-poor DNA in the male chromocenter, but equally distributed AT and GC DNA sequences in male and female euchromatin, like in T. infestans. Accumulation of argyrophilic proteins encircling the chromocenter did not always correlate with that of GC-rich DNA. Methylated DNA identified by immunodetection was found sparsely distributed in the euchromatin of both sexes and at some points around the chromocenter edge, but it could not be considered responsible for chromatin condensation in the chromocenter, like in T. infestans. However, unlike in T. infestans, no correlation between the chromocenter AT-rich DNA and nucleolus organizing region (NOR) DNA was found in P. megistus.
dc.description114
dc.description665-72
dc.languageeng
dc.relationActa Histochemica
dc.relationActa Histochem.
dc.rightsfechado
dc.rightsCopyright © 2011 Elsevier GmbH. All rights reserved.
dc.sourcePubMed
dc.subjectAt Rich Sequence
dc.subjectAnimals
dc.subjectCell Nucleus
dc.subjectCells, Cultured
dc.subjectChromatin
dc.subjectCytidine
dc.subjectDna Methylation
dc.subjectEpithelial Cells
dc.subjectFemale
dc.subjectGc Rich Sequence
dc.subjectGenes, Insect
dc.subjectMale
dc.subjectMalpighian Tubules
dc.subjectMice
dc.subjectNucleolus Organizer Region
dc.subjectNymph
dc.subjectPanstrongylus
dc.titleContribution Of At-, Gc-, And Methylated Cytidine-rich Dna To Chromatin Composition In Malpighian Tubule Cell Nuclei Of Panstrongylus Megistus (hemiptera, Reduviidae).
dc.typeArtículos de revistas


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