Exopolysaccharide Matrix Of Developed Candida Albicans Biofilms After Exposure To Antifungal Agents.

dc.creatorda Silva, Wander José
dc.creatorGonçalves, Letícia Machado
dc.creatorSeneviratne, Jayampath
dc.creatorParahitiyawa, Nipuna
dc.creatorSamaranayake, Lakshman Perera
dc.creatorDel Bel Cury, Altair Antoninha
dc.date2012
dc.date2015-11-27T13:29:09Z
dc.date2015-11-27T13:29:09Z
dc.date.accessioned2018-03-29T01:16:38Z
dc.date.available2018-03-29T01:16:38Z
dc.identifierBrazilian Dental Journal. v. 23, n. 6, p. 716-22, 2012.
dc.identifier1806-4760
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23338267
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200334
dc.identifier23338267
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300567
dc.descriptionThis study aimed to evaluate the effects of fluconazole or nystatin exposure on developed Candida albicans biofilms regarding their exopolysaccharide matrix. The minimal inhibitory concentration (MIC) against fluconazole or nystatin was determined for C. albicans reference strain (ATCC 90028). Poly(methlymethacrylate) resin (PMMA) specimens were fabricated according to the manufacturer's instructions and had their surface roughness measured. Biofilms were developed on specimens surfaces for 48 h and after that were exposed during 24 h to fluconazole or nystatin prepared in a medium at MIC, 10 x MIC or 100 x MIC. Metabolic activity was evaluated using an XTT assay. Production of soluble and insoluble exopolysaccharide and intracellular polysaccharides was evaluated by the phenol-sulfuric method. Confocal laser scanning microscope was used to evaluate biofilm architecture and percentage of dead/live cells. Data were analyzed statistically by ANOVA and Tukey's test at 5% significance level. The presence of fluconazole or nystatin at concentrations higher than MIC results in a great reduction of metabolic activity (p<0.001). At MIC or 10 x MIC, fluconazole showed high amounts of intracellular polysaccharides (p<0.05), but did not affect the exopolysaccharide matrix (p>0.05). The exposure to nystatin also did not alter the exopolysaccharide matrix at all the tested concentrations (p>0.05). Biofilm architecture was not affected by either of the antifungal agents (p>0.05). Nystatin promoted higher proportion of dead cells (p<0.05). It may be concluded that fluconazole and nystatin above the MIC concentration reduced the metabolic activity of C. albicans biofilms; however, they were not able to alter the exopolysaccharide matrix and biofilm architecture.
dc.description23
dc.description716-22
dc.languageeng
dc.relationBrazilian Dental Journal
dc.relationBraz Dent J
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAntifungal Agents
dc.subjectBiofilms
dc.subjectCandida Albicans
dc.subjectColorimetry
dc.subjectCulture Media
dc.subjectFluconazole
dc.subjectFungal Polysaccharides
dc.subjectHumans
dc.subjectHyphae
dc.subjectIndicators And Reagents
dc.subjectMicrobial Sensitivity Tests
dc.subjectMicrobial Viability
dc.subjectMicroscopy, Confocal
dc.subjectNystatin
dc.subjectPolymethyl Methacrylate
dc.subjectSolubility
dc.subjectSurface Properties
dc.subjectTetrazolium Salts
dc.subjectTime Factors
dc.titleExopolysaccharide Matrix Of Developed Candida Albicans Biofilms After Exposure To Antifungal Agents.
dc.typeArtículos de revistas


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