Quantitative Changes In Human Epithelial Cancers And Osteogenesis Imperfecta Disease Detected Using Nonlinear Multicontrast Microscopy.

dc.creatorAdur, Javier
dc.creatorPelegati, Vitor B
dc.creatorde Thomaz, Andre A
dc.creatorD'Souza-Li, Lilia
dc.creatorAssunção, Maria do Carmo
dc.creatorBottcher-Luiz, Fátima
dc.creatorAndrade, Liliana A L A
dc.creatorCesar, Carlos L
dc.date2012-Aug
dc.date2015-11-27T13:29:05Z
dc.date2015-11-27T13:29:05Z
dc.date.accessioned2018-03-29T01:16:29Z
dc.date.available2018-03-29T01:16:29Z
dc.identifierJournal Of Biomedical Optics. v. 17, n. 8, p. 081407-1, 2012-Aug.
dc.identifier1560-2281
dc.identifier10.1117/1.JBO.17.8.081407
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23224168
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200296
dc.identifier23224168
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300529
dc.descriptionWe show that combined multimodal nonlinear optical (NLO) microscopies, including two-photon excitation fluorescence, second-harmonic generation (SHG), third harmonic generation, and fluorescence lifetime imaging microscopy (FLIM) can be used to detect morphological and metabolic changes associated with stroma and epithelial transformation during the progression of cancer and osteogenesis imperfecta (OI) disease. NLO microscopes provide complementary information about tissue microstructure, showing distinctive patterns for different types of human breast cancer, mucinous ovarian tumors, and skin dermis of patients with OI. Using a set of scoring methods (anisotropy, correlation, uniformity, entropy, and lifetime components), we found significant differences in the content, distribution and organization of collagen fibrils in the stroma of breast and ovary as well as in the dermis of skin. We suggest that our results provide a framework for using NLO techniques as a clinical diagnostic tool for human cancer and OI. We further suggest that the SHG and FLIM metrics described could be applied to other connective or epithelial tissue disorders that are characterized by abnormal cells proliferation and collagen assembly.
dc.description17
dc.description081407-1
dc.languageeng
dc.relationJournal Of Biomedical Optics
dc.relationJ Biomed Opt
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAdult
dc.subjectAged
dc.subjectBreast Neoplasms
dc.subjectFemale
dc.subjectHumans
dc.subjectMicroscopy, Fluorescence, Multiphoton
dc.subjectMiddle Aged
dc.subjectNeoplasms, Glandular And Epithelial
dc.subjectNonlinear Dynamics
dc.subjectOsteogenesis Imperfecta
dc.subjectPrecancerous Conditions
dc.subjectReproducibility Of Results
dc.subjectSensitivity And Specificity
dc.titleQuantitative Changes In Human Epithelial Cancers And Osteogenesis Imperfecta Disease Detected Using Nonlinear Multicontrast Microscopy.
dc.typeArtículos de revistas


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