A Potential Role For An Extracellular Methanol Oxidase Secreted By Moniliophthora Perniciosa In Witches' Broom Disease In Cacao.

dc.creatorde Oliveira, Bruno V
dc.creatorTeixeira, Gleidson S
dc.creatorReis, Osvaldo
dc.creatorBarau, Joan G
dc.creatorTeixeira, Paulo José P L
dc.creatordo Rio, Maria Carolina S
dc.creatorDomingues, Romênia R
dc.creatorMeinhardt, Lyndel W
dc.creatorPaes Leme, Adriana F
dc.creatorRincones, Johana
dc.creatorPereira, Gonçalo A G
dc.date2012-Nov
dc.date2015-11-27T13:28:56Z
dc.date2015-11-27T13:28:56Z
dc.date.accessioned2018-03-29T01:16:10Z
dc.date.available2018-03-29T01:16:10Z
dc.identifierFungal Genetics And Biology : Fg & B. v. 49, n. 11, p. 922-32, 2012-Nov.
dc.identifier1096-0937
dc.identifier10.1016/j.fgb.2012.09.001
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/23022488
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200217
dc.identifier23022488
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300450
dc.descriptionThe hemibiotrophic basidiomycete fungus Moniliophthora perniciosa, the causal agent of Witches' broom disease (WBD) in cacao, is able to grow on methanol as the sole carbon source. In plants, one of the main sources of methanol is the pectin present in the structure of cell walls. Pectin is composed of highly methylesterified chains of galacturonic acid. The hydrolysis between the methyl radicals and galacturonic acid in esterified pectin, mediated by a pectin methylesterase (PME), releases methanol, which may be decomposed by a methanol oxidase (MOX). The analysis of the M. pernciosa genome revealed putative mox and pme genes. Real-time quantitative RT-PCR performed with RNA from mycelia grown in the presence of methanol or pectin as the sole carbon source and with RNA from infected cacao seedlings in different stages of the progression of WBD indicate that the two genes are coregulated, suggesting that the fungus may be metabolizing the methanol released from pectin. Moreover, immunolocalization of homogalacturonan, the main pectic domain that constitutes the primary cell wall matrix, shows a reduction in the level of pectin methyl esterification in infected cacao seedlings. Although MOX has been classically classified as a peroxisomal enzyme, M. perniciosa presents an extracellular methanol oxidase. Its activity was detected in the fungus culture supernatants, and mass spectrometry analysis indicated the presence of this enzyme in the fungus secretome. Because M. pernciosa possesses all genes classically related to methanol metabolism, we propose a peroxisome-independent model for the utilization of methanol by this fungus, which begins with the extracellular oxidation of methanol derived from the demethylation of pectin and finishes in the cytosol.
dc.description49
dc.description922-32
dc.languageeng
dc.relationFungal Genetics And Biology : Fg & B
dc.relationFungal Genet. Biol.
dc.rightsfechado
dc.rightsCopyright © 2012 Elsevier Inc. All rights reserved.
dc.sourcePubMed
dc.subjectAgaricales
dc.subjectAlcohol Oxidoreductases
dc.subjectAmino Acid Sequence
dc.subjectCacao
dc.subjectExtracellular Space
dc.subjectFungal Proteins
dc.subjectGene Expression Regulation, Fungal
dc.subjectMethanol
dc.subjectMolecular Sequence Data
dc.subjectPectins
dc.subjectPlant Diseases
dc.subjectProtein Transport
dc.subjectSequence Alignment
dc.titleA Potential Role For An Extracellular Methanol Oxidase Secreted By Moniliophthora Perniciosa In Witches' Broom Disease In Cacao.
dc.typeArtículos de revistas


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