dc.creatorGhiraldini, Flávia G
dc.creatorSilva, Isabela S
dc.creatorMello, Maria Luiza S
dc.date2012-Sep
dc.date2015-11-27T13:28:45Z
dc.date2015-11-27T13:28:45Z
dc.date.accessioned2018-03-29T01:15:49Z
dc.date.available2018-03-29T01:15:49Z
dc.identifierCytometry. Part A : The Journal Of The International Society For Analytical Cytology. v. 81, n. 9, p. 755-64, 2012-Sep.
dc.identifier1552-4930
dc.identifier10.1002/cyto.a.22102
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/22837107
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200125
dc.identifier22837107
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300358
dc.descriptionChanges in polyploidization, chromatin supraorganization, and chromatin accessibility were investigated in hepatocytes collected from adult, nonobese diabetic (NOD) mice with increasing hyperglycemia and compared with adult normoglycemic controls and 56-week-old normoglycemic BALB/c mice. Our goal was to determine the changes in ploidy degrees and chromatin characteristics in mouse hepatocytes that are associated with insulin-dependent diabetes and to detect similarities in these aspects with those verified with aging, with greater accuracy than previous studies. Image analysis of Feulgen-stained nuclei revealed changes in ploidy degrees and chromatin supraorganization. Chromatin accessibility was assessed with micrococcal nuclease (MNase) digestion. Increased polyploidy was associated with increasing levels of glycemia, and this trend toward polyploidy was found even under normoglycemic conditions in NOD mice. Although high degrees of ploidy were also detected in aged BALB/c mice, the magnitude of polyploidy was not the same magnitude as that in the diabetic mice. While there was increased homogeneity of chromatin packaging with increasing polyploidy under conditions of severe hyperglycemia (and even under conditions of normoglycemia) in NOD mice, an inverse relationship was observed in aged BALB/c mice. Chromatin accessibility to MNase increased under severe hyperglycemia and advanced age, but it was much higher in the diabetic mice. In conclusion, although similarities in polyploidy were observed between the hepatocytes from increasingly hyperglycemic adult mice and those from normoglycemic aged mice, the relationship between chromatin remodeling and increases in ploidy degrees was not the same between the hepatocytes of these two groups. These findings demonstrate that strict similarities between diabetes and aging are not always true at the cellular level. This discordance is likely due to differences in the metabolic state of mouse hepatocytes during aging and diabetic conditions consequent to specificities in their gene regulatory programs.
dc.description81
dc.description755-64
dc.languageeng
dc.relationCytometry. Part A : The Journal Of The International Society For Analytical Cytology
dc.relationCytometry A
dc.rightsfechado
dc.rightsCopyright © 2012 International Society for Advancement of Cytometry.
dc.sourcePubMed
dc.subjectAging
dc.subjectAnimals
dc.subjectBlood Glucose
dc.subjectChromatin
dc.subjectChromatin Assembly And Disassembly
dc.subjectColoring Agents
dc.subjectDna
dc.subjectDna Cleavage
dc.subjectDiabetes Mellitus, Type 1
dc.subjectFemale
dc.subjectHepatocytes
dc.subjectImage Processing, Computer-assisted
dc.subjectMice
dc.subjectMice, Inbred Balb C
dc.subjectMice, Inbred Nod
dc.subjectMicrococcal Nuclease
dc.subjectPolyploidy
dc.subjectRosaniline Dyes
dc.titlePolyploidy And Chromatin Remodeling In Hepatocytes From Insulin-dependent Diabetic And Normoglycemic Aged Mice.
dc.typeArtículos de revistas


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