Quercetin Decreases Inflammatory Response And Increases Insulin Action In Skeletal Muscle Of Ob/ob Mice And In L6 Myotubes.

dc.creatorAnhê, Gabriel F
dc.creatorOkamoto, Maristela M
dc.creatorKinote, Andrezza
dc.creatorSollon, Carolina
dc.creatorLellis-Santos, Camilo
dc.creatorAnhê, Fernando F
dc.creatorLima, Guilherme A
dc.creatorHirabara, Sandro M
dc.creatorVelloso, Licio A
dc.creatorBordin, Silvana
dc.creatorMachado, Ubiratan F
dc.date2012-Aug
dc.date2015-11-27T13:28:39Z
dc.date2015-11-27T13:28:39Z
dc.date.accessioned2018-03-29T01:15:35Z
dc.date.available2018-03-29T01:15:35Z
dc.identifierEuropean Journal Of Pharmacology. v. 689, n. 1-3, p. 285-93, 2012-Aug.
dc.identifier1879-0712
dc.identifier10.1016/j.ejphar.2012.06.007
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/22713545
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200067
dc.identifier22713545
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300300
dc.descriptionQuercetin is a potent anti-inflammatory flavonoid, but its capacity to modulate insulin sensitivity in obese insulin resistant conditions is unknown. This study investigated the effect of quercetin treatment upon insulin sensitivity of ob/ob mice and its potential molecular mechanisms. Obese ob/ob mice were treated with quercetin for 10 weeks, and L6 myotubes were treated with either palmitate or tumor necrosis factor-α (TNFα) plus quercetin. Cells and muscles were processed for analysis of glucose transporter 4 (GLUT4), TNFα and inducible nitric oxide synthase (iNOS) expression, and c-Jun N-terminal kinase (JNK) and inhibitor of nuclear factor-κB (NF-κB) kinase (IκK) phosphorylation. Myotubes were assayed for glucose uptake and NF-κB translocation. Chromatin immunoprecipitation assessed NF-κB binding to GLUT4 promoter. Quercetin treatment improved whole body insulin sensitivity by increasing GLUT4 expression and decreasing JNK phosphorylation, and TNFα and iNOS expression in skeletal muscle. Quercetin suppressed palmitate-induced upregulation of TNFα and iNOS and restored normal levels of GLUT4 in myotubes. In parallel, quercetin suppressed TNFα-induced reduction of glucose uptake in myotubes. Nuclear accumulation of NF-κB in myotubes and binding of NF-κB to GLUT4 promoter in muscles of ob/ob mice were also reduced by quercetin. We demonstrated that quercetin decreased the inflammatory status in skeletal muscle of obese mice and in L6 myotubes. This effect was followed by increased muscle GLUT4, with parallel improvement of insulin sensitivity. These results point out quercetin as a putative strategy to manage inflammatory-related insulin resistance.
dc.description689
dc.description285-93
dc.languageeng
dc.relationEuropean Journal Of Pharmacology
dc.relationEur. J. Pharmacol.
dc.rightsfechado
dc.rightsCopyright © 2012 Elsevier B.V. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAntioxidants
dc.subjectDown-regulation
dc.subjectInflammation
dc.subjectInflammation Mediators
dc.subjectInsulin
dc.subjectMale
dc.subjectMice
dc.subjectMice, Obese
dc.subjectMuscle Fibers, Skeletal
dc.subjectMuscle, Skeletal
dc.subjectQuercetin
dc.subjectUp-regulation
dc.titleQuercetin Decreases Inflammatory Response And Increases Insulin Action In Skeletal Muscle Of Ob/ob Mice And In L6 Myotubes.
dc.typeArtículos de revistas


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