Guanidine Affects Differentially The Twitch Response Of Diaphragm, Extensor Digitorum Longus And Soleus Nerve-muscle Preparations Of Mice.

dc.creatorFerrari, Rosana
dc.creatorRodrigues-Simioni, Léa
dc.creatorda Cruz Höfling, Maria Alice
dc.date2012
dc.date2015-11-27T13:28:38Z
dc.date2015-11-27T13:28:38Z
dc.date.accessioned2018-03-29T01:15:34Z
dc.date.available2018-03-29T01:15:34Z
dc.identifierMolecules (basel, Switzerland). v. 17, n. 6, p. 7503-22, 2012.
dc.identifier1420-3049
dc.identifier10.3390/molecules17067503
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/22706376
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200062
dc.identifier22706376
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300295
dc.descriptionGuanidine has been used with some success to treat myasthenia gravis and myasthenic syndrome because it increases acetylcholine release at nerve terminals through K⁺, Na⁺ and Ca²⁺ channels-involving mechanisms. Currently, guanidine derivatives have been proposed for treatment of several diseases. Studies aimed at providing new insights to the drug are relevant. Experimentally, guanidine (10 mM) induces on mouse phrenic nerve-diaphragm (PND) preparations neurotransmission facilitation followed by blockade and a greatest secondary facilitation after its removal from bath. Herein, we hypothesized that this peculiar triphasic response may differ in muscles with distinct twitch/metabolic characteristics. Morphological alterations and contractile response of PND, extensor digitorum longus (EDL) and soleus (SOL) preparations incubated with guanidine (10 mM) for 15, 30, 60 min were analyzed. Guanidine concentrations of 5 mM (for PND and EDL) and 1 mM (for EDL) were also tested. Guanidine triphasic effect was only observed on PND regardless the concentration. The morphological alterations in muscle tissue varied along time but did not impede the PND post-wash facilitation. Higher doses (20-25 mM) did not increase EDL or SOL neurotransmission. The data suggest a complex mechanism likely dependent on the metabolic/contractile muscle phenotype; muscle fiber types and density/type of ion channels, sarcoplasmic reticulum and mitochondria organization may have profound impact on the levels and isoform expression pattern of Ca²⁺ regulatory membrane proteins so reflecting regulation of calcium handling and contractile response in different types of muscle.
dc.description17
dc.description7503-22
dc.languageeng
dc.relationMolecules (basel, Switzerland)
dc.relationMolecules
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCalcium
dc.subjectDiaphragm
dc.subjectGuanidine
dc.subjectMale
dc.subjectMice
dc.subjectMuscle Contraction
dc.subjectMuscle, Skeletal
dc.subjectMyography
dc.subjectNeuromuscular Junction
dc.subjectSynaptic Transmission
dc.titleGuanidine Affects Differentially The Twitch Response Of Diaphragm, Extensor Digitorum Longus And Soleus Nerve-muscle Preparations Of Mice.
dc.typeArtículos de revistas


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