Quantification Of Cultivable Bacteria And Endotoxin In Post-treatment Apical Periodontitis Before And After Chemo-mechanical Preparation.

dc.creatorEndo, M S
dc.creatorMartinho, F C
dc.creatorZaia, A A
dc.creatorFerraz, C C R
dc.creatorAlmeida, J F A
dc.creatorGomes, B P F A
dc.date2012-Oct
dc.date2015-11-27T13:28:30Z
dc.date2015-11-27T13:28:30Z
dc.date.accessioned2018-03-29T01:15:18Z
dc.date.available2018-03-29T01:15:18Z
dc.identifierEuropean Journal Of Clinical Microbiology & Infectious Diseases : Official Publication Of The European Society Of Clinical Microbiology. v. 31, n. 10, p. 2575-83, 2012-Oct.
dc.identifier1435-4373
dc.identifier10.1007/s10096-012-1598-6
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/22565224
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199997
dc.identifier22565224
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300230
dc.descriptionThis clinical study was conducted to quantify cultivable bacteria and endotoxin in root canals with post-treatment apical periodontitis by correlating their levels with clinical features and to evaluate the effect of chemo-mechanical preparation (CMP) with 2 % chlorhexidine gel + 17 % EDTA on bacterial and endotoxin removal/elimination. Moreover, target strict Gram-negative anaerobic bacteria were investigated by polymerase chain reaction (PCR). Fifteen teeth with post-treatment apical periodontitis were sampled before (s1) and after (s2) CMP. Culture techniques determined the number of colony-forming units (CFU). PCR (16S rDNA) and limulus amebocyte lysate (LAL) assay were used for bacterial and endotoxin detection, respectively. Prevotella nigrescens (4/15), Prevotella intermedia (2/15), and Tannerella forsythia (2/15) were the most frequently detected species. Endotoxin was recovered in 100 % of the samples. At s1, bacteria and endotoxin were detected at a median value of 5.14 × 10(3) CFU/mL and 3.96 EU/mL, respectively. Higher levels of endotoxin were related to a larger size of radiolucent area (>5 mm) (p < 0.05). CMP was more effective in reducing bacteria (99.61 %) than endotoxin (60.6 %) (both p < 0.05). Our findings indicated that the levels of endotoxin found in infected root canals were related to a larger size of radiolucent area in the periapical region. Moreover, CMP was effective in reducing both bacterial and endotoxin contents in post-treatment apical periodontitis.
dc.description31
dc.description2575-83
dc.languageeng
dc.relationEuropean Journal Of Clinical Microbiology & Infectious Diseases : Official Publication Of The European Society Of Clinical Microbiology
dc.relationEur. J. Clin. Microbiol. Infect. Dis.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnti-bacterial Agents
dc.subjectBacterial Load
dc.subjectBacterial Typing Techniques
dc.subjectChlorhexidine
dc.subjectDental Pulp Cavity
dc.subjectEdetic Acid
dc.subjectEndotoxins
dc.subjectHumans
dc.subjectMicrobial Viability
dc.subjectPeriapical Periodontitis
dc.subjectPolymerase Chain Reaction
dc.subjectPrevotella
dc.subjectRna, Bacterial
dc.subjectRna, Ribosomal, 16s
dc.subjectTreatment Outcome
dc.subjectTreponema
dc.titleQuantification Of Cultivable Bacteria And Endotoxin In Post-treatment Apical Periodontitis Before And After Chemo-mechanical Preparation.
dc.typeArtículos de revistas


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