Human Sepsis-associated Escherichia Coli (sepec) Is Able To Adhere To And Invade Kidney Epithelial Cells In Culture.

dc.creatorConceição, R A
dc.creatorLudovico, M S
dc.creatorAndrade, C G T J
dc.creatorYano, T
dc.date2012-May
dc.date2015-11-27T13:28:24Z
dc.date2015-11-27T13:28:24Z
dc.date.accessioned2018-03-29T01:15:09Z
dc.date.available2018-03-29T01:15:09Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 45, n. 5, p. 417-24, 2012-May.
dc.identifier1414-431X
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/22488222
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199956
dc.identifier22488222
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1300189
dc.descriptionThe adhesins of extraintestinal pathogenic Escherichia coli are essential for mediating direct interactions between the microbes and the host cell surfaces that they infect. Using fluorescence microscopy and gentamycin protection assays, we observed that 49 sepsis-associated E. coli (SEPEC) strains isolated from human adults adhered to and invaded Vero cells in the presence of D-mannose (100%). In addition, bacteria concentrations of approximately 2 x 10(7) CFU/mL were recovered from Vero cells following an invasion assay. Furthermore, PCR analysis of adhesin genes showed that 98.0% of these SEPEC strains tested positive for fimH, 69.4% for flu, 53.1% for csgA, 38.8% for mat, and 32.7% for iha. Analysis of the invasin genes showed that 16.3% of the SEPEC strains were positive for tia, 12.3% for gimB, and 10.2% for ibeA. Therefore, these data suggest that SEPEC adhesion to cell surfaces occurs through non-fimH mechanisms. Scanning electron microscopy showed the formation of microcolonies on the Vero cell surface. SEPEC invasiveness was also confirmed by the presence of intracellular bacteria, and ultrastructural analysis using electron transmission microscopy revealed bacteria inside the Vero cells. Taken together, these results demonstrate that these SEPEC strains had the ability to adhere to and invade Vero cells. Moreover, these data support the theory that renal cells may be the predominant pathway through which SEPEC enters human blood vessels.
dc.description45
dc.description417-24
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAdhesins, Bacterial
dc.subjectAdult
dc.subjectAnimals
dc.subjectBacterial Adhesion
dc.subjectCercopithecus Aethiops
dc.subjectEpithelial Cells
dc.subjectEscherichia Coli
dc.subjectEscherichia Coli Infections
dc.subjectGenotype
dc.subjectHumans
dc.subjectMicroscopy, Electron, Scanning
dc.subjectMicroscopy, Fluorescence
dc.subjectPolymerase Chain Reaction
dc.subjectSepsis
dc.subjectVero Cells
dc.titleHuman Sepsis-associated Escherichia Coli (sepec) Is Able To Adhere To And Invade Kidney Epithelial Cells In Culture.
dc.typeArtículos de revistas


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