Correlation Between Api 50 Ch And Multiplex Polymerase Chain Reaction For The Identification Of Vaginal Lactobacilli In Isolates.

dc.creatorBrolazo, Eliane Melo
dc.creatorLeite, Domingos Silva
dc.creatorTiba, Monique Ribeiro
dc.creatorVillarroel, Marina
dc.creatorMarconi, Camila
dc.creatorSimoes, Jose Antonio
dc.date2011-Jan
dc.date2015-11-27T13:22:11Z
dc.date2015-11-27T13:22:11Z
dc.date.accessioned2018-03-29T01:14:26Z
dc.date.available2018-03-29T01:14:26Z
dc.identifierBrazilian Journal Of Microbiology : [publication Of The Brazilian Society For Microbiology]. v. 42, n. 1, p. 225-32, 2011-Jan.
dc.identifier1517-8382
dc.identifier10.1590/S1517-83822011000100028
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24031625
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199765
dc.identifier24031625
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299998
dc.descriptionIdentification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability of the epidemiological and probiotics studies. Therefore this study aimed to determine the best methodology for the identification of the large diversity of lactobacilli species found in the vagina by comparing two techniques, one based on their biochemical profile and other employing molecular biology. A carbohydrate fermentation test (API 50 CH) was compared with multiplex polymerase chain reaction (PCR) for the identification of species of vaginal lactobacilli from 135 healthy women. The kappa index was used to evaluate agreement between the methods. Using the molecular technique, L. crispatus (32.6%), L. jensenii (25%) and L. gasseri (20.6%) were the most frequent species. However, using the biochemical technique, the most frequent species were: L. acidophilus (34.8%), L. crispatus (27.2%) and L. fermentum (13%). Although L. acidophilus was the most frequent specie found by biochemical tests, no strain of this microorganism was detected by PCR. Agreement between the methods was low for identification of all the most common species. Although rates of L. crispatus detected were similar using both methods (32.6% and 27.2%), agreement between them was relatively low (kappa = 0.52). Our results confirmed the limitation of the biochemical method and the applicability of a previously published molecular method (Multiplex PCR) for the identification of lactobacilli in the vaginal tract, focusing on further necessity of its improvement for also targeting L. vaginalis and L. iners.
dc.description42
dc.description225-32
dc.languageeng
dc.relationBrazilian Journal Of Microbiology : [publication Of The Brazilian Society For Microbiology]
dc.relationBraz. J. Microbiol.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectCarbohydrate Fermentation Test
dc.subjectIdentification
dc.subjectMultiplex Pcr
dc.subjectVaginal Ecosystem
dc.subjectVaginal Lactobacilli
dc.titleCorrelation Between Api 50 Ch And Multiplex Polymerase Chain Reaction For The Identification Of Vaginal Lactobacilli In Isolates.
dc.typeArtículos de revistas


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