The Small Heat Shock Proteins From Acidithiobacillus Ferrooxidans: Gene Expression, Phylogenetic Analysis, And Structural Modeling.

dc.creatorRibeiro, Daniela A
dc.creatorBem, Luiz E V Del
dc.creatorVicentini, Renato
dc.creatorFerraz, Lúcio F C
dc.creatorMurakami, Mario T
dc.creatorOttoboni, Laura M M
dc.date2011
dc.date2015-11-27T13:22:04Z
dc.date2015-11-27T13:22:04Z
dc.date.accessioned2018-03-29T01:14:12Z
dc.date.available2018-03-29T01:14:12Z
dc.identifierBmc Microbiology. v. 11, p. 259, 2011.
dc.identifier1471-2180
dc.identifier10.1186/1471-2180-11-259
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/22151959
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199708
dc.identifier22151959
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299941
dc.descriptionAcidithiobacillus ferrooxidans is an acidophilic, chemolithoautotrophic bacterium that has been successfully used in metal bioleaching. In this study, an analysis of the A. ferrooxidans ATCC 23270 genome revealed the presence of three sHSP genes, Afe_1009, Afe_1437 and Afe_2172, that encode proteins from the HSP20 family, a class of intracellular multimers that is especially important in extremophile microorganisms. The expression of the sHSP genes was investigated in A. ferrooxidans cells submitted to a heat shock at 40°C for 15, 30 and 60 minutes. After 60 minutes, the gene on locus Afe_1437 was about 20-fold more highly expressed than the gene on locus Afe_2172. Bioinformatic and phylogenetic analyses showed that the sHSPs from A. ferrooxidans are possible non-paralogous proteins, and are regulated by the σ32 factor, a common transcription factor of heat shock proteins. Structural studies using homology molecular modeling indicated that the proteins encoded by Afe_1009 and Afe_1437 have a conserved α-crystallin domain and share similar structural features with the sHSP from Methanococcus jannaschii, suggesting that their biological assembly involves 24 molecules and resembles a hollow spherical shell. We conclude that the sHSPs encoded by the Afe_1437 and Afe_1009 genes are more likely to act as molecular chaperones in the A. ferrooxidans heat shock response. In addition, the three sHSPs from A. ferrooxidans are not recent paralogs, and the Afe_1437 and Afe_1009 genes could be inherited horizontally by A. ferrooxidans.
dc.description11
dc.description259
dc.languageeng
dc.relationBmc Microbiology
dc.relationBMC Microbiol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAcidithiobacillus
dc.subjectAmino Acid Sequence
dc.subjectBacterial Proteins
dc.subjectBase Sequence
dc.subjectComputational Biology
dc.subjectGene Expression Regulation, Bacterial
dc.subjectHeat-shock Proteins, Small
dc.subjectHot Temperature
dc.subjectModels, Molecular
dc.subjectMolecular Sequence Data
dc.subjectPhylogeny
dc.subjectProtein Structure, Tertiary
dc.subjectSequence Analysis, Protein
dc.titleThe Small Heat Shock Proteins From Acidithiobacillus Ferrooxidans: Gene Expression, Phylogenetic Analysis, And Structural Modeling.
dc.typeArtículos de revistas


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