Sugarcane Hsp101 Is A Hexameric Chaperone That Binds Nucleotides.

dc.creatorCagliari, Thiago C
dc.creatorda Silva, Viviane C H
dc.creatorBorges, Júlio C
dc.creatorPrando, Alessandra
dc.creatorTasic, Ljubica
dc.creatorRamos, Carlos H I
dc.date2011-Dec
dc.date2015-11-27T13:21:52Z
dc.date2015-11-27T13:21:52Z
dc.date.accessioned2018-03-29T01:13:54Z
dc.date.available2018-03-29T01:13:54Z
dc.identifierInternational Journal Of Biological Macromolecules. v. 49, n. 5, p. 1022-30, 2011-Dec.
dc.identifier1879-0003
dc.identifier10.1016/j.ijbiomac.2011.08.027
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/21903129
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199627
dc.identifier21903129
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299860
dc.descriptionThe Clp/Hsp100 AAA+ chaperone family is involved in recovering aggregated proteins and little is known about other orthologs of the well studied ClpB from Escherichia coli and Hsp104 from Saccharomyces cerevisiae. Plant Hsp101 is a good model for understanding the relationship between the structure and function of Hsp100 proteins and to investigate the role of these chaperones in disaggregation processes. Here, we present the cloning and purification of a sugarcane ortholog, SHsp101, which is expressed in sugarcane cells and is a folded hexamer that is capable of binding nucleotides. Thus SHsp101 has the structural and functional characteristics of the Clp/Hsp100 AAA+ family.
dc.description49
dc.description1022-30
dc.languageeng
dc.relationInternational Journal Of Biological Macromolecules
dc.relationInt. J. Biol. Macromol.
dc.rightsfechado
dc.rightsCopyright © 2011 Elsevier B.V. All rights reserved.
dc.sourcePubMed
dc.subjectAmino Acid Sequence
dc.subjectChimera
dc.subjectCloning, Molecular
dc.subjectEscherichia Coli
dc.subjectEscherichia Coli Proteins
dc.subjectHeat-shock Proteins
dc.subjectMagnetic Resonance Spectroscopy
dc.subjectModels, Molecular
dc.subjectMolecular Chaperones
dc.subjectMolecular Sequence Data
dc.subjectNucleotides
dc.subjectPlant Proteins
dc.subjectPlasmids
dc.subjectPolymerization
dc.subjectProtein Binding
dc.subjectProtein Folding
dc.subjectProtein Structure, Tertiary
dc.subjectRecombinant Proteins
dc.subjectSaccharomyces Cerevisiae
dc.subjectSaccharomyces Cerevisiae Proteins
dc.subjectSaccharum
dc.subjectStructure-activity Relationship
dc.subjectTranscription Factors
dc.subjectUltracentrifugation
dc.titleSugarcane Hsp101 Is A Hexameric Chaperone That Binds Nucleotides.
dc.typeArtículos de revistas


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