Sildenafil Preserves Diastolic Relaxation After Reduction By L-name And Increases Phosphodiesterase-5 In The Intercalated Discs Of Cardiac Myocytes And Arterioles.

dc.creatorFerreira-Melo, Silvia Elaine
dc.creatorDemacq, Caroline
dc.creatorLacchini, Silvia
dc.creatorKrieger, José Eduardo
dc.creatorIrigoyen, Maria Cláudia
dc.creatorMoreno, Heitor
dc.date2011
dc.date2015-11-27T13:21:50Z
dc.date2015-11-27T13:21:50Z
dc.date.accessioned2018-03-29T01:13:49Z
dc.date.available2018-03-29T01:13:49Z
dc.identifierClinics (são Paulo, Brazil). v. 66, n. 7, p. 1253-8, 2011.
dc.identifier1980-5322
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/21876983
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199611
dc.identifier21876983
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299844
dc.descriptionWe investigated the influence of sildenafil on cardiac contractility and diastolic relaxation and examined the distribution of phosphodiesterase-5 in the hearts of hypertensive rats that were treated with by NG-nitro-L-arginine methyl ester (L-NAME). Male Wistar rats were treated with L-NAME and/or sildenafil for eight weeks. The Langendorff method was used to examine the effects of sildenafil on cardiac contractility and diastolic relaxation. The presence and location of phosphodiesterase-5 and phosphodiesterase-3 were assessed by immunohistochemistry, and cGMP plasma levels were measured by ELISA. In isolated hearts, sildenafil prevented the reduction of diastolic relaxation (dP/dt) that was induced by L-NAME. In addition, phosphodiesterase-5 immunoreactivity was localized in the intercalated discs between the myocardial cells. The staining intensity was reduced by L-NAME, and sildenafil treatment abolished this reduction. Consistent with these results, the plasma levels of cGMP were decreased in the L-NAME-treated rats but not in rats that were treated with L-NAME + sildenafil. The sildenafil-induced attenuation of the deleterious hemodynamic and cardiac morphological effects of L-NAME in cardiac myocytes is mediated (at least in part) by the inhibition of phosphodiesterase-5.
dc.description66
dc.description1253-8
dc.languageeng
dc.relationClinics (são Paulo, Brazil)
dc.relationClinics (Sao Paulo)
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectArterioles
dc.subjectBlood Pressure
dc.subjectCyclic Nucleotide Phosphodiesterases, Type 5
dc.subjectDiastole
dc.subjectEnzyme Inhibitors
dc.subjectEnzyme-linked Immunosorbent Assay
dc.subjectHeart
dc.subjectHypertension
dc.subjectMale
dc.subjectMyocytes, Cardiac
dc.subjectNg-nitroarginine Methyl Ester
dc.subjectPhosphodiesterase 5 Inhibitors
dc.subjectPiperazines
dc.subjectPurines
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSulfones
dc.subjectTime Factors
dc.titleSildenafil Preserves Diastolic Relaxation After Reduction By L-name And Increases Phosphodiesterase-5 In The Intercalated Discs Of Cardiac Myocytes And Arterioles.
dc.typeArtículos de revistas


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