Osteopontin, A Chemotactic Protein With Cytokine-like Properties, Is Up-regulated In Muscle Injury Caused By Bothrops Lanceolatus (fer-de-lance) Snake Venom.

dc.creatorBarbosa-Souza, Valéria
dc.creatorContin, Daniel Kiss
dc.creatorFilho, Waldemar Bonventi
dc.creatorde Araújo, Albetiza Lôbo
dc.creatorIrazusta, Silvia Pierre
dc.creatorda Cruz-Höfling, Maria Alice
dc.date2011-Oct
dc.date2015-11-27T13:21:46Z
dc.date2015-11-27T13:21:46Z
dc.date.accessioned2018-03-29T01:13:43Z
dc.date.available2018-03-29T01:13:43Z
dc.identifierToxicon : Official Journal Of The International Society On Toxinology. v. 58, n. 5, p. 398-409, 2011-Oct.
dc.identifier1879-3150
dc.identifier10.1016/j.toxicon.2011.07.011
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/21839764
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199584
dc.identifier21839764
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299817
dc.descriptionOsteopontin (OPN) is a chemotactic, adhesive protein whose receptors include some integrins and matrix proteins known to have role in inflammatory and repair processes. We examined the time course of OPN expression at acute and chronic stages after intramuscular injection of Bothrops lanceolatus venom in rats. Additionally, we examined the expression of CD68 (a marker for phagocytic macrophages) and the myogenic factors, myoD and myogenin. There was a biphasic upregulation of OPN (6-48 h and 3-14 days post-venom), i.e., during acute inflammation and myogenic cell proliferation and differentiation phases. OPN was detected in CD68 + macrophages, fibroblasts, normal and damaged myofibers, myoblasts and myotubes. Myogenin was expressed in the cytoplasm (atypical pattern) and nucleus of myoblasts and myotubes from 18 h to 7 days, after which it was expressed only in nuclei. Macrophage numbers, OPN and myogenin expression were still elevated at 7, 14 and 7 days. At 3 days, when OPN achieved the peak, some clusters of myoblasts were within regions of intense collagen deposition. Fibrosis may represent limitation for repairing processes and may explain the small diameter of regenerated fibers at 21 days post-venom. The expression of OPN in the course of venom-induced damage and regeneration suggests stages-specific mediation role along the whole process.
dc.description58
dc.description398-409
dc.languageeng
dc.relationToxicon : Official Journal Of The International Society On Toxinology
dc.relationToxicon
dc.rightsfechado
dc.rightsCopyright © 2011 Elsevier Ltd. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAntigens, Cd
dc.subjectAntigens, Differentiation, Myelomonocytic
dc.subjectBothrops
dc.subjectCell Proliferation
dc.subjectCrotalid Venoms
dc.subjectImmunohistochemistry
dc.subjectMuscles
dc.subjectOsteopontin
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectUp-regulation
dc.titleOsteopontin, A Chemotactic Protein With Cytokine-like Properties, Is Up-regulated In Muscle Injury Caused By Bothrops Lanceolatus (fer-de-lance) Snake Venom.
dc.typeArtículos de revistas


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