Supraorganized Collagen Enhances Schwann Cell Reactivity And Organization In Vitro.

dc.creatorMaturana, L G
dc.creatorZanon, R G
dc.creatorPierucci, A
dc.creatorVidal, B C
dc.creatorOliveira, A L R
dc.date2011-Jul
dc.date2015-11-27T13:21:45Z
dc.date2015-11-27T13:21:45Z
dc.date.accessioned2018-03-29T01:13:42Z
dc.date.available2018-03-29T01:13:42Z
dc.identifierBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 44, n. 7, p. 682-7, 2011-Jul.
dc.identifier1414-431X
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/21833459
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199577
dc.identifier21833459
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299810
dc.descriptionWe investigated the reactivity and expression of basal lamina collagen by Schwann cells (SCs) cultivated on a supraorganized bovine-derived collagen substrate. SC cultures were obtained from sciatic nerves of neonatal Sprague-Dawley rats and seeded on 24-well culture plates containing collagen substrate. The homogeneity of the cultures was evaluated with an SC marker antibody (anti-S-100). After 1 week, the cultures were fixed and processed for immunocytochemistry by using antibodies against type IV collagen, S-100 and p75NTR (pan neurotrophin receptor) and for scanning electron microscopy (SEM). Positive labeling with antibodies to the cited molecules was observed, indicating that the collagen substrate stimulates SC alignment and adhesion (collagen IV labeling - organized collagen substrate: 706.33 ± 370.86, non-organized collagen substrate: 744.00 ± 262.09; S-100 labeling - organized collagen: 3809.00 ± 120.28, non-organized collagen: 3026.00 ± 144.63, P < 0.05) and reactivity (p75NTR labeling - organized collagen: 2156.33 ± 561.78, non-organized collagen: 1424.00 ± 405.90, P < 0.05; means ± standard error of the mean in absorbance units). Cell alignment and adhesion to the substrate were confirmed by SEM analysis. The present results indicate that the collagen substrate with an aligned suprastructure, as seen by polarized light microscopy, provides an adequate scaffold for SCs, which in turn may increase the efficiency of the nerve regenerative process after in vivo repair.
dc.description44
dc.description682-7
dc.languageeng
dc.relationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]
dc.relationBraz. J. Med. Biol. Res.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCattle
dc.subjectCell Polarity
dc.subjectCell Shape
dc.subjectCells, Cultured
dc.subjectCollagen Type Iv
dc.subjectExtracellular Matrix
dc.subjectImmunohistochemistry
dc.subjectMaterials Testing
dc.subjectMicroscopy, Electron, Scanning
dc.subjectNerve Regeneration
dc.subjectPolymers
dc.subjectRats
dc.subjectRats, Sprague-dawley
dc.subjectReceptors, Nerve Growth Factor
dc.subjectS100 Proteins
dc.subjectSchwann Cells
dc.subjectSciatic Nerve
dc.subjectStaining And Labeling
dc.titleSupraorganized Collagen Enhances Schwann Cell Reactivity And Organization In Vitro.
dc.typeArtículos de revistas


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