Apoptotic Ratios And Mitotic Abnormalities In 17-β-estradiol-transformed Human Breast Epithelial Mcf-10f Cells.

dc.creatorCruz, L M S
dc.creatorFerreira, J C V
dc.creatorMello, M L S
dc.date2011-May
dc.date2015-11-27T13:21:39Z
dc.date2015-11-27T13:21:39Z
dc.date.accessioned2018-03-29T01:13:31Z
dc.date.available2018-03-29T01:13:31Z
dc.identifierBrazilian Journal Of Biology = Revista Brasleira De Biologia. v. 71, n. 2, p. 487-90, 2011-May.
dc.identifier1678-4375
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/21755167
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199527
dc.identifier21755167
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299760
dc.descriptionTreatment of human breast epithelial cells MCF-10F with 17-β-estradiol has been reported to result in E2-transformed cells which have given rise to highly invasive C5 cells that in turn generate tumors in SCID mice. From these tumors, various cell lines, among which C5-A6-T6 and C5-A8-T8, were obtained. Although different phases of the tumorigenesis process in this model have been studied in molecular biology and image analysis assays, no cytological data on apoptotic ratios and mitotic abnormalities have been established to accompany the various steps leading to 17-β-estradiol-treated MCF-10F cells to tumorigenesis. Here we detected that the apoptotic ratio decreases with the transformation and tumorigenesis progress, except for the tumor cell line C5-A8-T8, probably on account of its more intense proliferation rate and a more rapid culture medium consumption. Increased frequency of mitotic abnormalities contributed by triple- and tetrapolar metaphases, and by lagging chromosomes and chromosome bridges observed at the anaphase found by transformation and tumorigenesis progress. However, no difference was found under these terms when the C5-A6-T6 and C5-A8-T8 tumor cell lines were compared to each other. Present findings are in agreement with the nuclear instability and enrichment of dysregulated genes in the apoptotic process promoted by transformation and tumorigenesis in 17-β-estradiol-treated MCF-10F cells.
dc.description71
dc.description487-90
dc.languageeng
dc.relationBrazilian Journal Of Biology = Revista Brasleira De Biologia
dc.relationBraz J Biol
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectBreast Neoplasms
dc.subjectCell Line, Transformed
dc.subjectCell Line, Tumor
dc.subjectCell Transformation, Neoplastic
dc.subjectEpithelial Cells
dc.subjectEstradiol
dc.subjectFemale
dc.subjectHumans
dc.subjectMice
dc.subjectMitosis
dc.titleApoptotic Ratios And Mitotic Abnormalities In 17-β-estradiol-transformed Human Breast Epithelial Mcf-10f Cells.
dc.typeArtículos de revistas


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