Molecular Characterization Of A Miraculin-like Gene Differentially Expressed During Coffee Development And Coffee Leaf Miner Infestation.

dc.creatorMondego, Jorge Maurício Costa
dc.creatorDuarte, Melina Pasini
dc.creatorKiyota, Eduardo
dc.creatorMartínez, Leandro
dc.creatorde Camargo, Sandra Rodrigues
dc.creatorDe Caroli, Fernanda P
dc.creatorAlves, Beatriz Santos Capela
dc.creatorGuerreiro, Sandra Maria Carmello
dc.creatorOliva, Maria Luiza Vilela
dc.creatorGuerreiro-Filho, Oliveiro
dc.creatorMenossi, Marcelo
dc.date2011-Jan
dc.date2015-11-27T13:21:38Z
dc.date2015-11-27T13:21:38Z
dc.date.accessioned2018-03-29T01:13:28Z
dc.date.available2018-03-29T01:13:28Z
dc.identifierPlanta. v. 233, n. 1, p. 123-37, 2011-Jan.
dc.identifier1432-2048
dc.identifier10.1007/s00425-010-1284-9
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20931223
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199520
dc.identifier20931223
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299753
dc.descriptionThe characterization of a coffee gene encoding a protein similar to miraculin-like proteins, which are members of the plant Kunitz serine trypsin inhibitor (STI) family of proteinase inhibitors (PIs), is described. PIs are important proteins in plant defence against insects and in the regulation of proteolysis during plant development. This gene has high identity with the Richadella dulcifica taste-modifying protein miraculin and with the tomato protein LeMir; and was named as CoMir (Coffea miraculin). Structural protein modelling indicated that CoMir had structural similarities with the Kunitz STI proteins, but suggested specific folding structures. CoMir was up-regulated after coffee leaf miner (Leucoptera coffella) oviposition in resistant plants of a progeny derived from crosses between C. racemosa (resistant) and C. arabica (susceptible). Interestingly, this gene was down-regulated during coffee leaf miner herbivory in susceptible plants. CoMir expression was up-regulated after abscisic acid application and wounding stress and was prominent during the early stages of flower and fruit development. In situ hybridization revealed that CoMir transcripts accumulated in the anther tissues that display programmed cell death (tapetum, endothecium and stomium) and in the metaxylem vessels of the petals, stigma and leaves. In addition, the recombinant protein CoMir shows inhibitory activity against trypsin. According to the present results CoMir may act in proteolytic regulation during coffee development and in the defence against L. coffeella. The similarity of CoMir with other Kunitz STI proteins and the role of CoMir in plant development and plant stress are discussed.
dc.description233
dc.description123-37
dc.languageeng
dc.relationPlanta
dc.relationPlanta
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectBlotting, Southern
dc.subjectCoffee
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation, Developmental
dc.subjectGene Expression Regulation, Plant
dc.subjectGenes, Plant
dc.subjectGlycoproteins
dc.subjectModels, Molecular
dc.subjectMolecular Sequence Data
dc.subjectMoths
dc.subjectOrgan Specificity
dc.subjectPhylogeny
dc.subjectPlant Proteins
dc.subjectProtein Processing, Post-translational
dc.subjectRna, Messenger
dc.subjectSequence Alignment
dc.subjectSequence Analysis, Dna
dc.titleMolecular Characterization Of A Miraculin-like Gene Differentially Expressed During Coffee Development And Coffee Leaf Miner Infestation.
dc.typeArtículos de revistas


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