Application Of Electrochemically Produced Aluminum Hydroxide Gel For Prepurification Of Recombinant Synthetic Green Fluorescent Protein Produced In Tobacco Leaves.

dc.creatorRobić, Goran
dc.creatorLacorte, Cristiano
dc.creatorRech, Elibio L
dc.creatorMiranda, Everson A
dc.date2011-Jul
dc.date2015-11-27T13:21:29Z
dc.date2015-11-27T13:21:29Z
dc.date.accessioned2018-03-29T01:13:13Z
dc.date.available2018-03-29T01:13:13Z
dc.identifierBiotechnology Progress. v. 27, n. 4, p. 1029-35, 2011-Jul.
dc.identifier1520-6033
dc.identifier10.1002/btpr.627
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/21626720
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199456
dc.identifier21626720
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299689
dc.descriptionThe use of recombinant proteins has increased greatly in recent years, as also have increased the number of techniques and materials used for their production and purification. Among the different types of bioreactors being studied, there is a general consensus among scientists that production in green plant tissues such as leaves is more feasible. However, the presence of chlorophyll and phenolic compounds in plant extracts, which can precipitate and denature the proteins besides damaging separation membranes and gels, makes this technology impracticable on a commercial scale. In the present work, the adsorption to electrochemically produced aluminum hydroxide gel was applied as a prepurification step for recombinant synthetic green fluorescent protein (sGFP), also referred to as enhanced green fluorescent protein, produced in Nicotiana benthamiana leaves. Removal efficiencies of 99.7% of chlorophyll, 88.5% of phenolic compounds, and 38.5% of native proteins from the N. benthamiana extracts were achieved without removing sGFP from the extracts. As electrochemical preparation of aluminum hydroxide gel is a cost-effective technique, its use can substantially contribute to the development of future production platforms for recombinant proteins produced in green plant tissues of pharmaceutical and industrial interest.
dc.description27
dc.description1029-35
dc.languageeng
dc.relationBiotechnology Progress
dc.relationBiotechnol. Prog.
dc.rightsfechado
dc.rightsCopyright © 2011 American Institute of Chemical Engineers (AIChE).
dc.sourcePubMed
dc.subjectAluminum Hydroxide
dc.subjectChlorophyll
dc.subjectGreen Fluorescent Proteins
dc.subjectIsoelectric Focusing
dc.subjectPhenols
dc.subjectPlant Extracts
dc.subjectPlant Leaves
dc.subjectPlants, Genetically Modified
dc.subjectRecombinant Proteins
dc.subjectTobacco
dc.titleApplication Of Electrochemically Produced Aluminum Hydroxide Gel For Prepurification Of Recombinant Synthetic Green Fluorescent Protein Produced In Tobacco Leaves.
dc.typeArtículos de revistas


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