Enzyme Characterisation, Isolation And Cdna Cloning Of Polyphenol Oxidase In The Hearts Of Palm Of Three Commercially Important Species.

dc.creatorShimizu, Milton Massao
dc.creatorMelo, Geraldo Aclécio
dc.creatorBrombini Dos Santos, Adriana
dc.creatorBottcher, Alexandra
dc.creatorCesarino, Igor
dc.creatorAraújo, Pedro
dc.creatorMagalhães Silva Moura, Jullyana Cristina
dc.creatorMazzafera, Paulo
dc.date2011-Sep
dc.date2015-11-27T13:21:21Z
dc.date2015-11-27T13:21:21Z
dc.date.accessioned2018-03-29T01:13:01Z
dc.date.available2018-03-29T01:13:01Z
dc.identifierPlant Physiology And Biochemistry : Ppb / Société Française De Physiologie Végétale. v. 49, n. 9, p. 970-7, 2011-Sep.
dc.identifier1873-2690
dc.identifier10.1016/j.plaphy.2011.04.006
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/21530289
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199402
dc.identifier21530289
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299635
dc.descriptionHeart of palm (palmito) is the edible part of the apical meristem of palms and is considered a gourmet vegetable. Palmitos from the palms Euterpe edulis (Juçara) and Euterpe oleracea (Açaí) oxidise after harvesting, whereas almost no oxidation is observed in palmitos from Bactris gasipaes (Pupunha). Previous investigations showed that oxidation in Juçara and Açaí was mainly attributable to polyphenol oxidase (PPO; EC 1.14.18.1) activity. In this study, we partially purified PPOs from these three palmitos and analysed them for SDS activation, substrate specificity, inhibition by specific inhibitors, thermal stability, optimum pH and temperature conditions, Km and Ki. In addition, the total phenolic content and chlorogenic acid content were determined. Two partial cDNA sequences were isolated and sequenced from Açaí (EoPPO1) and Juçara (EePPO1). Semi-quantitative RT-PCR expression assays showed that Açaí and Juçara PPOs were strongly expressed in palmitos and weakly expressed in leaves. No amplification was observed for Pupunha samples. The lack of oxidation in the palmito Pupunha might be explained by the low PPO expression, low enzyme activity or the phenolic profile, particularly the low content of chlorogenic acid.
dc.description49
dc.description970-7
dc.languageeng
dc.relationPlant Physiology And Biochemistry : Ppb / Société Française De Physiologie Végétale
dc.relationPlant Physiol. Biochem.
dc.rightsfechado
dc.rightsCopyright © 2011 Elsevier Masson SAS. All rights reserved.
dc.sourcePubMed
dc.subjectArecaceae
dc.subjectCatechol Oxidase
dc.subjectCloning, Molecular
dc.subjectDna, Complementary
dc.subjectEnzyme Inhibitors
dc.subjectEnzyme Stability
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectGene Expression Regulation, Plant
dc.subjectHydrogen-ion Concentration
dc.subjectPlant Proteins
dc.subjectSubstrate Specificity
dc.titleEnzyme Characterisation, Isolation And Cdna Cloning Of Polyphenol Oxidase In The Hearts Of Palm Of Three Commercially Important Species.
dc.typeArtículos de revistas


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