Satellite Dna Derived From 5s Rdna In Physalaemus Cuvieri (anura, Leiuperidae).

dc.creatorVittorazzi, S E
dc.creatorLourenço, L B
dc.creatorDel-Grande, M L
dc.creatorRecco-Pimentel, S M
dc.date2011
dc.date2015-11-27T13:21:15Z
dc.date2015-11-27T13:21:15Z
dc.date.accessioned2018-03-29T01:12:51Z
dc.date.available2018-03-29T01:12:51Z
dc.identifierCytogenetic And Genome Research. v. 134, n. 2, p. 101-7, 2011.
dc.identifier1424-859X
dc.identifier10.1159/000325540
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/21464559
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199357
dc.identifier21464559
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299590
dc.descriptionIn the present study, we describe for the first time a family of 190-bp satellite DNA related to 5S rDNA in anurans and the existence of 2 forms of 5S rDNA, type I (201 bp) and type II (690 bp). The sequences were obtained from genomic DNA of Physalaemus cuvieri from Palmeiras, State of Bahia, Brazil. Analysis of the nucleotide sequence revealed that the satellite DNA obtained by digestion with EcoRI, called PcP190EcoRI, is 70% similar to the coding region of type I 5S rDNA and 66% similar to the coding region of type II 5S rDNA. Membrane hybridization and PCR amplification of the sequence showed that PcP190EcoRI is tandemly repeated. The satellite DNA as well as type I and type II 5S rDNA were localized in P. cuvieri chromosomes by fluorescent in situ hybridization. The PcP190EcoRI sequence was found in the centromeres of chromosomes 1-5 and in the pericentromeric region of chromosome 3. Type I 5S rDNA was detected in chromosome 3, coincident with the site of PcP190EcoRI. Type II 5S rDNA was located interstitially in the long arm of chromosome 5. None of these sequences co-localized with nucleolar organizer regions. Our data suggests that this satellite DNA originates from the 5S ribosomal multigene family, probably by gene duplication, nucleotide divergence and sequence dispersion in the genome.
dc.description134
dc.description101-7
dc.languageeng
dc.relationCytogenetic And Genome Research
dc.relationCytogenet. Genome Res.
dc.rightsfechado
dc.rightsCopyright © 2011 S. Karger AG, Basel.
dc.sourcePubMed
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectAnura
dc.subjectDna, Ribosomal
dc.subjectDna, Satellite
dc.subjectFemale
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectKaryotyping
dc.subjectMale
dc.subjectMolecular Sequence Data
dc.subjectSequence Alignment
dc.titleSatellite Dna Derived From 5s Rdna In Physalaemus Cuvieri (anura, Leiuperidae).
dc.typeArtículos de revistas


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