Profiling The Changes In Signaling Pathways In Ascorbic Acid/β-glycerophosphate-induced Osteoblastic Differentiation.

dc.creatorChaves Neto, Antonio Hernandes
dc.creatorQueiroz, Karla Cristiana
dc.creatorMilani, Renato
dc.creatorParedes-Gamero, Edgar Julian
dc.creatorJusto, Giselle Zenker
dc.creatorPeppelenbosch, Maikel P
dc.creatorFerreira, Carmen Veríssima
dc.date2011-Jan
dc.date2015-11-27T13:21:14Z
dc.date2015-11-27T13:21:14Z
dc.date.accessioned2018-03-29T01:12:50Z
dc.date.available2018-03-29T01:12:50Z
dc.identifierJournal Of Cellular Biochemistry. v. 112, n. 1, p. 71-7, 2011-Jan.
dc.identifier1097-4644
dc.identifier10.1002/jcb.22763
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20626033
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199355
dc.identifier20626033
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299588
dc.descriptionDespite numerous reports on the ability of ascorbic acid and β-glycerophosphate (AA/β-GP) to induce osteoblast differentiation, little is known about the molecular mechanisms involved in this phenomenon. In this work, we used a peptide array containing specific consensus sequences (potential substrates) for protein kinases and traditional biochemical techniques to examine the signaling pathways modulated during AA/β-GP-induced osteoblast differentiation. The kinomic profile obtained after 7 days of treatment with AA/β-GP identified 18 kinase substrates with significantly enhanced or reduced phosphorylation. Peptide substrates for Akt, PI3K, PKC, BCR, ABL, PRKG1, PAK1, PAK2, ERK1, ERBB2, and SYK showed a considerable reduction in phosphorylation, whereas enhanced phosphorylation was observed in substrates for CHKB, CHKA, PKA, FAK, ATM, PKA, and VEGFR-1. These findings confirm the potential usefulness of peptide microarrays for identifying kinases known to be involved in bone development in vivo and in vitro and show that this technique can be used to investigate kinases whose function in osteoblastic differentiation is poorly understood.
dc.description112
dc.description71-7
dc.languageeng
dc.relationJournal Of Cellular Biochemistry
dc.relationJ. Cell. Biochem.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectAscorbic Acid
dc.subjectCell Differentiation
dc.subjectCells, Cultured
dc.subjectGlycerophosphates
dc.subjectMice
dc.subjectOsteoblasts
dc.subjectPhosphorylation
dc.subjectSignal Transduction
dc.titleProfiling The Changes In Signaling Pathways In Ascorbic Acid/β-glycerophosphate-induced Osteoblastic Differentiation.
dc.typeArtículos de revistas


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