Extended Chromatin Fibers And Chromatin Organization.

dc.creatorMello, M L S
dc.creatorMoraes, A S
dc.creatorVidal, B C
dc.date2011-Aug
dc.date2015-11-27T13:20:58Z
dc.date2015-11-27T13:20:58Z
dc.date.accessioned2018-03-29T01:12:26Z
dc.date.available2018-03-29T01:12:26Z
dc.identifierBiotechnic & Histochemistry : Official Publication Of The Biological Stain Commission. v. 86, n. 4, p. 213-25, 2011-Aug.
dc.identifier1473-7760
dc.identifier10.3109/10520290903549022
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20105097
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199253
dc.identifier20105097
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299486
dc.descriptionStudies of chromatin extensibility have revealed the flow of chromatin and DNA from cell nuclei and chromosomes in response to gravity or mechanical stretch following lysis by hypertonic saline and detergent solutions. Since this phenomenon was first reported, the technical methods by which extended chromatin fibers (ECFs) may be analyzed have been improved. These methods include topochemical assays, fluorescence in situ hybridization, immunofluorescence, electron microscopy, and polarization microscopy. Chromatin and DNA halos also have been studied in materials subjected to lysis, especially in a horizontal position or after cytocentrifugation. The analysis of ECF formation is useful not only as a tool for detecting the positioning of certain DNA signals on chromatin filaments, but also for describing diverse DNA-protein associations that may be related to varying transcriptional activities and chromatin supraorganization. A brief review of the methods and applications of ECF formation is presented here. We focus on light microscopy studies of ECF formation in mouse hepatocytes under different chromatin supraorganization and physiological conditions and in sperm cells with different DNA-protein complexes.
dc.description86
dc.description213-25
dc.languageeng
dc.relationBiotechnic & Histochemistry : Official Publication Of The Biological Stain Commission
dc.relationBiotech Histochem
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBirefringence
dc.subjectCell Nucleus
dc.subjectChromatin
dc.subjectChromosomes
dc.subjectDna
dc.subjectFemale
dc.subjectFluorescent Antibody Technique
dc.subjectHepatocytes
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMale
dc.subjectMice
dc.subjectMicroscopy, Electron
dc.subjectNuclear Matrix
dc.subjectSpermatozoa
dc.titleExtended Chromatin Fibers And Chromatin Organization.
dc.typeArtículos de revistas


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