Single Embryo And Oocyte Lipid Fingerprinting By Mass Spectrometry.

dc.creatorFerreira, Christina R
dc.creatorSaraiva, Sergio A
dc.creatorCatharino, Rodrigo R
dc.creatorGarcia, Jerusa S
dc.creatorGozzo, Fabio C
dc.creatorSanvido, Gustavo B
dc.creatorSantos, Luiz Fernando A
dc.creatorLo Turco, Edson G
dc.creatorPontes, José Henrique F
dc.creatorBasso, Andréa C
dc.creatorBertolla, Ricardo P
dc.creatorSartori, Roberto
dc.creatorGuardieiro, Monique M
dc.creatorPerecin, Felipe
dc.creatorMeirelles, Flávio V
dc.creatorSangalli, Juliano R
dc.creatorEberlin, Marcos N
dc.date2010-May
dc.date2015-11-27T13:18:37Z
dc.date2015-11-27T13:18:37Z
dc.date.accessioned2018-03-29T01:12:17Z
dc.date.available2018-03-29T01:12:17Z
dc.identifierJournal Of Lipid Research. v. 51, n. 5, p. 1218-27, 2010-May.
dc.identifier0022-2275
dc.identifier10.1194/jlr.D001768
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19965589
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199216
dc.identifier19965589
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299449
dc.descriptionMethods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid (represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species.
dc.description51
dc.description1218-27
dc.languageeng
dc.relationJournal Of Lipid Research
dc.relationJ. Lipid Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCattle
dc.subjectEmbryo, Mammalian
dc.subjectEmbryonic Development
dc.subjectFemale
dc.subjectHumans
dc.subjectLipids
dc.subjectOocytes
dc.subjectSpecies Specificity
dc.subjectSpectrometry, Mass, Matrix-assisted Laser Desorption-ionization
dc.titleSingle Embryo And Oocyte Lipid Fingerprinting By Mass Spectrometry.
dc.typeArtículos de revistas


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