Brasil | Artículos de revistas

Release Of Cytokines By Stimulated Peripheral Blood Mononuclear Cells In Chronic Periodontitis.

dc.creatorGonçalves, Thais Oliveira
dc.creatorCosta, Dirceu
dc.creatorBrodskyn, Claudia Ida
dc.creatorDuarte, Poliana Mendes
dc.creatorCésar Neto, João Batista
dc.creatorNogueira-Filho, Getulio
dc.date2010-Dec
dc.date2015-11-27T13:18:15Z
dc.date2015-11-27T13:18:15Z
dc.date.accessioned2018-03-29T01:11:38Z
dc.date.available2018-03-29T01:11:38Z
dc.identifierArchives Of Oral Biology. v. 55, n. 12, p. 975-80, 2010-Dec.
dc.identifier1879-1506
dc.identifier10.1016/j.archoralbio.2010.08.002
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20880516
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199043
dc.identifier20880516
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299276
dc.descriptionThe emergence of periodontal medicine increased interest in defining the behaviour of peripheral blood cells in periodontitis subjects in comparison with healthy group. The aim of this study was to evaluate the levels of interleukin (IL)-8, tumour necrosis factor-α (TNF-α), IL-6 and IL-10 released by Escherichia coli lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMC) obtained from the peripheral blood of chronic periodontitis subjects. PBMC samples were isolated from 19 systemically healthy donors, divided into generalized chronic periodontitis (n=10) and healthy (n=9) subjects. Cells were incubated for 24-48 h in 500 μL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS. Supernatants were used to quantify the amounts of IL-8, TNF-α, IL-6 and IL-10 released using enzyme-linked immunosorbent assay (ELISA). PBMC cells from periodontitis subjects released higher levels of TNF-α and IL-6 than those from healthy subjects (P<0.05). Conversely, the supernatants of the stimulated PBMC cells obtained from healthy subjects presented higher amounts of IL-8 than those from periodontitis (P<0.05). No differences were observed in the levels of IL-10 (P>0.05) between groups. In conclusion, the results of the present study showed that E. coli LPS-stimulated PBMC from subjects with periodontitis present a different pattern of cytokine release when compared to PBMC from healthy subjects. This phenomenon could have implications locally, in periodontitis, as well as in systemic diseases.
dc.description55
dc.description975-80
dc.languageeng
dc.relationArchives Of Oral Biology
dc.relationArch. Oral Biol.
dc.rightsfechado
dc.rightsCopyright © 2010 Elsevier Ltd. All rights reserved.
dc.sourcePubMed
dc.subjectAdult
dc.subjectAged
dc.subjectCells, Cultured
dc.subjectChronic Periodontitis
dc.subjectCytokines
dc.subjectEscherichia Coli
dc.subjectFemale
dc.subjectGingival Hemorrhage
dc.subjectHumans
dc.subjectInflammation Mediators
dc.subjectInterleukin-10
dc.subjectInterleukin-6
dc.subjectInterleukin-8
dc.subjectLeukocytes, Mononuclear
dc.subjectLipopolysaccharides
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectPeriodontal Attachment Loss
dc.subjectPeriodontal Pocket
dc.subjectSmoking
dc.subjectTumor Necrosis Factor-alpha
dc.titleRelease Of Cytokines By Stimulated Peripheral Blood Mononuclear Cells In Chronic Periodontitis.
dc.typeArtículos de revistas


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