Hypoxia, Hypoxia-inducible Factor-1α And Vascular Endothelial Growth Factor In A Murine Model Of Schistosoma Mansoni Infection.

dc.creatorAraújo, Alexandra Paiva
dc.creatorFrezza, Tarsila Ferraz
dc.creatorAllegretti, Silmara Marques
dc.creatorGiorgio, Selma
dc.date2010-Dec
dc.date2015-11-27T13:18:13Z
dc.date2015-11-27T13:18:13Z
dc.date.accessioned2018-03-29T01:11:36Z
dc.date.available2018-03-29T01:11:36Z
dc.identifierExperimental And Molecular Pathology. v. 89, n. 3, p. 327-33, 2010-Dec.
dc.identifier1096-0945
dc.identifier10.1016/j.yexmp.2010.09.003
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20858486
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199031
dc.identifier20858486
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299264
dc.descriptionSchistosomiasis mansoni is a chronic parasitic disease where much of the symptomatology is attributed to granuloma formation, an immunopathological reaction against Schistosoma eggs. To more clearly understand the immunopathology of schistosomiasis, the tissue microenvironment generated by S. mansoni infected mice was investigated. Using the hypoxia marker pimonidazole, we provide immunohistochemical evidence that hypoxia occurred in inflammatory cells infiltrated around the eggs and cells surrounding granulomas in the liver, intestine, spleen and lungs of infected mice. Hypoxia-inducible factor-1α (HIF-1α) was mainly expressed in inflammatory cells surrounding the eggs and in hepatocytes surrounding cellular and fibrocellular granulomas in infected mouse liver. HIF-1α expression was also verified in granulomas in the other tissues tested (intestine, spleen and lungs). Vascular endothelial growth factor (VEGF) expression was observed in the extracellular space surrounding inflammatory cells in liver granuloma. The VEGF expression pattern verified in infected mouse liver was very similar to that observed in the other tissues tested. A strong positive correlation occurred between pimonidazole binding and HIF-1α and VEGF expression in the tissues tested, except for lung. This work is the first evidence that infection by a helminth parasite, S. mansoni, produces a hypoxic tissue microenvironment and induces HIF-1α and VEGF expression.
dc.description89
dc.description327-33
dc.languageeng
dc.relationExperimental And Molecular Pathology
dc.relationExp. Mol. Pathol.
dc.rightsfechado
dc.rightsCopyright © 2010 Elsevier Inc. All rights reserved.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCell Hypoxia
dc.subjectDisease Models, Animal
dc.subjectFemale
dc.subjectGranuloma
dc.subjectHypoxia-inducible Factor 1, Alpha Subunit
dc.subjectImmunohistochemistry
dc.subjectMice
dc.subjectSchistosomiasis Mansoni
dc.subjectVascular Endothelial Growth Factor A
dc.titleHypoxia, Hypoxia-inducible Factor-1α And Vascular Endothelial Growth Factor In A Murine Model Of Schistosoma Mansoni Infection.
dc.typeArtículos de revistas


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