The Type Vi Secretion System Plays A Role In Type 1 Fimbria Expression And Pathogenesis Of An Avian Pathogenic Escherichia Coli Strain.

dc.creatorde Pace, Fernanda
dc.creatorNakazato, Gerson
dc.creatorPacheco, Alline
dc.creatorde Paiva, Jacqueline Boldrin
dc.creatorSperandio, Vanessa
dc.creatorda Silveira, Wanderley Dias
dc.date2010-Dec
dc.date2015-11-27T13:18:13Z
dc.date2015-11-27T13:18:13Z
dc.date.accessioned2018-03-29T01:11:35Z
dc.date.available2018-03-29T01:11:35Z
dc.identifierInfection And Immunity. v. 78, n. 12, p. 4990-8, 2010-Dec.
dc.identifier1098-5522
dc.identifier10.1128/IAI.00531-10
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20855516
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199029
dc.identifier20855516
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299262
dc.descriptionAvian pathogenic Escherichia coli (APEC) strains frequently cause extraintestinal infections and are responsible for significant economic losses in the poultry industry worldwide. APEC isolates are closely related to human extraintestinal pathogenic E. coli (ExPEC) strains and may also act as pathogens for humans. Known APEC virulence factors include adhesins such as type 1 fimbriae and curli, iron acquisition systems, and cytotoxins. Here we show that APEC strain SEPT362, isolated from a septicemic hen, expresses a type VI secretion system (T6SS); causes cytoskeleton rearrangements; and invades epithelial cells, replicates within macrophages, and causes lethal disease in chicks. To assess the contribution of the T6SS to SEPT362 pathogenesis, we generated two mutants, hcp (which encodes a protein suggested to be both secreted and a structural component of the T6SS) and clpV (encoding the T6SS ATPase). Both mutants showed decreased adherence and actin rearrangement on epithelial cells. However, only the hcp mutant presented a mild decrease in its ability to invade epithelial cells, and none of these mutants were defective for intramacrophage replication. Transcriptome studies showed that the level of expression of type 1 fimbriae was decreased in these mutants, which may account for the diminished adhesion and invasion of epithelial cells. The T6SS seems to be important for the disease process, given that both mutants were attenuated for infection in chicks. These results suggest that the T6SS influences the expression of type 1 fimbriae and contributes to APEC pathogenesis.
dc.description78
dc.description4990-8
dc.languageeng
dc.relationInfection And Immunity
dc.relationInfect. Immun.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectBacterial Adhesion
dc.subjectBacterial Secretion Systems
dc.subjectChickens
dc.subjectEscherichia Coli
dc.subjectEscherichia Coli Infections
dc.subjectFimbriae, Bacterial
dc.subjectGene Expression Regulation, Bacterial
dc.subjectHela Cells
dc.subjectHumans
dc.subjectMutation
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectPoultry Diseases
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectSepsis
dc.titleThe Type Vi Secretion System Plays A Role In Type 1 Fimbria Expression And Pathogenesis Of An Avian Pathogenic Escherichia Coli Strain.
dc.typeArtículos de revistas


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