Low-intensity Ultrasound Increases Fak, Erk-1/2, And Irs-1 Expression Of Intact Rat Bones In A Noncumulative Manner.

dc.creatorde Gusmão, Carlos Vinícius Buarque
dc.creatorPauli, José Rodrigo
dc.creatorSaad, Mario José Abdalla
dc.creatorAlves, José Marcos
dc.creatorBelangero, William Dias
dc.date2010-Apr
dc.date2015-11-27T13:18:12Z
dc.date2015-11-27T13:18:12Z
dc.date.accessioned2018-03-29T01:11:34Z
dc.date.available2018-03-29T01:11:34Z
dc.identifierClinical Orthopaedics And Related Research. v. 468, n. 4, p. 1149-56, 2010-Apr.
dc.identifier1528-1132
dc.identifier10.1007/s11999-009-1146-6
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19851814
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199023
dc.identifier19851814
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299256
dc.descriptionLow-intensity pulsed ultrasound stimulation (LIPUS) reportedly increases osteogenesis in fracture models but fails in intact bone, suggesting LIPUS does not act on mechanotransduction and growth factor pathways of intact bone. We asked whether daily 20-minute LIPUS applied to intact tibias would act on bone proteins involved in mechanotransduction (focal adhesion kinase [FAK], and extracellular signal-regulated kinase-1/2 [ERK-1/2]), and growth factor signaling (insulin receptor substrate-1 [IRS-1]) pathways at 7, 14, and 21 days of treatment. Immunoblotting was performed to detect FAK, ERK-1/2, and IRS-1 expression and activation from the stimulated intact tibias at 7, 14, and 21 days of daily 20-minute LIPUS. LIPUS increased FAK expression (at 7 days), ERK-1/2 (at 14 days), and IRS-1 (at 7 days), but expression decreased 7 days later, indicating a noncumulative effect of LIPUS. As only FAK expression was detected at 21 days, these observations suggest LIPUS influences nuclear reactions that may be modulated by a major cellular mechanism preferentially inhibiting IRS-1 expression and not FAK expression. Increased ERK-1/2 expression at 14 days suggests the differing mechanisms for promoting ERK-1/2, FAK, and IRS-1 syntheses. IRS-1 expression behaved similarly to FAK expression; therefore, LIPUS may modulate growth factor pathways. LIPUS increased sustained FAK and ERK-1/2 activation, but not IRS-1, suggesting sustained ERK-1/2 activation is not the result of mechanically induced growth factor activation. LIPUS acts on mechanotransduction and growth factor pathways in intact bone in a noncumulative manner. Clinical relevance These data suggest LIPUS applied to intact bone acts on proteins involved in osteogenesis.
dc.description468
dc.description1149-56
dc.languageeng
dc.relationClinical Orthopaedics And Related Research
dc.relationClin. Orthop. Relat. Res.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAnimals
dc.subjectExtracellular Signal-regulated Map Kinases
dc.subjectFocal Adhesion Protein-tyrosine Kinases
dc.subjectImmunoblotting
dc.subjectInsulin Receptor Substrate Proteins
dc.subjectMale
dc.subjectMechanotransduction, Cellular
dc.subjectMitogen-activated Protein Kinase 1
dc.subjectMitogen-activated Protein Kinase 3
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.subjectTibia
dc.subjectUltrasonic Therapy
dc.titleLow-intensity Ultrasound Increases Fak, Erk-1/2, And Irs-1 Expression Of Intact Rat Bones In A Noncumulative Manner.
dc.typeArtículos de revistas


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