Involvement Of Regulatory T Cells In The Immunosuppression Characteristic Of Patients With Paracoccidioidomycosis.

dc.creatorFerreira, Maria Carolina
dc.creatorde Oliveira, Rômulo Tadeu Dias
dc.creatorda Silva, Rosiane Maria
dc.creatorBlotta, Maria Heloisa Souza Lima
dc.creatorMamoni, Ronei Luciano
dc.date2010-Oct
dc.date2015-11-27T13:18:05Z
dc.date2015-11-27T13:18:05Z
dc.date.accessioned2018-03-29T01:11:18Z
dc.date.available2018-03-29T01:11:18Z
dc.identifierInfection And Immunity. v. 78, n. 10, p. 4392-401, 2010-Oct.
dc.identifier1098-5522
dc.identifier10.1128/IAI.00487-10
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20643858
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198960
dc.identifier20643858
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299193
dc.descriptionPatients with paracoccidioidomycosis (PCM) exhibit a suppression of the cellular immune response characterized by negative delayed-type hypersensitivity (DTH) to Paracoccidioides brasiliensis antigens, the apoptosis of lymphocytes, and high levels of expression of cytotoxic-T-lymphocyte-associated antigen 4 (CTLA-4), interleukin-10 (IL-10), and transforming growth factor β (TGF-β). The aim of this study was to investigate whether and how regulatory T cells (Treg cells) are involved in this immunosuppression by analyzing the number, phenotype, and activity of these cells in patients with active disease (AD group) and patients who had received treatment (TD group). Our results showed that the AD patients had more Treg cells than the TD patients or controls (C group) and also had elevated levels of expression of regulatory markers (glucocorticoid-induced tumor necrosis factor [TNF] receptor-related protein [GITR], CTLA-4, CD95L, LAP-1, and CD38). An analysis of regulatory activity showed that Treg cells from the AD group had greater activity than did cells from the other groups and that cell-cell contact is mandatory for this activity in the C group but was only partially involved in the regulatory activity of cells from AD patients. The addition of anti-IL-10 and anti-TGF-β neutralizing antibodies to the cultures showed that the production of cytokines may be another mechanism used by Treg cells. In conclusion, the elevated numbers of these cells with an increased regulatory phenotype and strong suppressive activity suggest a potential role for them in the immunosuppression characteristic of paracoccidioidomycosis. In addition, our results indicate that while Treg cells act by cell-cell contact, cytokine production also plays an important role.
dc.description78
dc.description4392-401
dc.languageeng
dc.relationInfection And Immunity
dc.relationInfect. Immun.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectAntifungal Agents
dc.subjectCoculture Techniques
dc.subjectCytokines
dc.subjectGene Expression Regulation
dc.subjectHumans
dc.subjectImmunity, Cellular
dc.subjectImmunocompromised Host
dc.subjectParacoccidioidomycosis
dc.subjectRna, Messenger
dc.subjectT-lymphocyte Subsets
dc.subjectT-lymphocytes, Regulatory
dc.titleInvolvement Of Regulatory T Cells In The Immunosuppression Characteristic Of Patients With Paracoccidioidomycosis.
dc.typeArtículos de revistas


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