Evolution On Experimental Animal Model For Upper Urothelium Carcinogenesis.

dc.creatorReis, Leonardo O
dc.creatorFávaro, Wagner J
dc.creatorFerreira, Ubirajara
dc.creatorBillis, Athanase
dc.creatorFazuoli, Mariana G
dc.creatorCagnon, Valéria H A
dc.date2010-Aug
dc.date2015-11-27T13:17:46Z
dc.date2015-11-27T13:17:46Z
dc.date.accessioned2018-03-29T01:10:42Z
dc.date.available2018-03-29T01:10:42Z
dc.identifierWorld Journal Of Urology. v. 28, n. 4, p. 499-505, 2010-Aug.
dc.identifier1433-8726
dc.identifier10.1007/s00345-010-0545-3
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20373103
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198805
dc.identifier20373103
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1299038
dc.descriptionNo optimal, well designed and reproducible animal model for upper urothelial carcinogenesis exists. This study characterized the histopathological features on top of immunolocalization of alpha-dystroglycans (alpha-DG) and matrix metalloproteinase (MMP-9) and cell turn-over in the upper urinary tract using a novel experimental model. Seventy-five female Fischer 344 rats were divided into three groups: the control group received a 0.30-ml dose of 0.9% physiological saline; the MNU group (chemical carcinogen N-methyl-N-nitrosourea) received 0.30 ml of MNU; and the MNU-citrate group received 0.30 ml of MNU plus sodium citrate, every one intravesically every other week for a total of 4 doses. After 15 weeks, bladder, ureters and renal pelvis were collected for morphological and molecular analysis. Associated management with MNU and sodium citrate was able to lead to 100% of both urinary bladder and upper urinary tract tumors, being the high-grade noninvasive papillary urothelial carcinoma the most frequent lesion. The upper urothelium showed reduced alpha-DG and increased MMP-9 and Ki-67 immunoreactivities in the MNU-citrate group in relation to the other groups. MNU group presented no upper urothelium tumor and 100% bladder tumor. This is a relevant evolution on experimental animal model for upper urinary tract carcinogenesis field. MMP-dependent disruption of the DG complex plays an important role in urothelial tumor carcinogenesis and showed the model applicability and significance. MNU-citrate model could contribute to a better understanding of human upper urothelial cancer development as well as to its local treatment strategies in a near future.
dc.description28
dc.description499-505
dc.languageeng
dc.relationWorld Journal Of Urology
dc.relationWorld J Urol
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAlkylating Agents
dc.subjectAnimals
dc.subjectBiopsy
dc.subjectCitrates
dc.subjectDisease Models, Animal
dc.subjectFemale
dc.subjectKidney Medulla
dc.subjectMethylnitrosourea
dc.subjectRats
dc.subjectRats, Inbred F344
dc.subjectUreter
dc.subjectUrinary Bladder
dc.subjectUrography
dc.subjectUrologic Neoplasms
dc.subjectUrothelium
dc.titleEvolution On Experimental Animal Model For Upper Urothelium Carcinogenesis.
dc.typeArtículos de revistas


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