Mmp-2 Regulates Rat Ventral Prostate Development In Vitro.

dc.creatorBruni-Cardoso, Alexandre
dc.creatorRosa-Ribeiro, Rafaela
dc.creatorPascoal, Vinicius D B
dc.creatorDe Thomaz, Andre A
dc.creatorCesar, Carlos L
dc.creatorCarvalho, Hernandes F
dc.date2010-Mar
dc.date2015-11-27T13:17:29Z
dc.date2015-11-27T13:17:29Z
dc.date.accessioned2018-03-29T01:10:17Z
dc.date.available2018-03-29T01:10:17Z
dc.identifierDevelopmental Dynamics : An Official Publication Of The American Association Of Anatomists. v. 239, n. 3, p. 737-46, 2010-Mar.
dc.identifier1097-0177
dc.identifier10.1002/dvdy.22222
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20108352
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198696
dc.identifier20108352
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298929
dc.descriptionWe have hypothesized that epithelial growth, branching, and canalization in the rodent ventral prostate (VP) would require matrix remodeling, and hence matrix metalloproteinase (MMP) activity. Therefore, the aim of this study was to evaluate the impact of blocking MMP-2, using whole organ culture. siRNA was employed to inhibit MMP-2 expression, and this was compared to GM6001's (a broad-spectrum MMP inhibitor) inhibition of general MMPs. These blocks impaired VP morphogenesis. MMP-2 silencing reduced organ size, epithelial area, and the number of tips, as well as caused a dilation of the distal parts of the epithelium. Histology, 3-D reconstruction, biochemistry, and second harmonic generation (SHG) revealed that MMP-2 silencing affected VP architecture by interfering in epithelial cell proliferation, lumen formation, and cellular organization of both epithelium and stroma, besides intense accumulation of collagen fibers. These data suggest that MMP-2 plays important roles in prostate growth, being directly involved with epithelial morphogenesis.
dc.description239
dc.description737-46
dc.languageeng
dc.relationDevelopmental Dynamics : An Official Publication Of The American Association Of Anatomists
dc.relationDev. Dyn.
dc.rightsfechado
dc.rightsCopyright (c) 2010 Wiley-Liss, Inc.
dc.sourcePubMed
dc.subjectAnimals
dc.subjectCell Proliferation
dc.subjectCollagen
dc.subjectEpithelium
dc.subjectGene Expression Regulation, Developmental
dc.subjectGene Silencing
dc.subjectImaging, Three-dimensional
dc.subjectIn Vitro Techniques
dc.subjectMale
dc.subjectMatrix Metalloproteinase 2
dc.subjectProstate
dc.subjectRna Processing, Post-transcriptional
dc.subjectRna, Small Interfering
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.titleMmp-2 Regulates Rat Ventral Prostate Development In Vitro.
dc.typeArtículos de revistas


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