Production, Purification And Application Of Extracellular Chitinase From Cellulosimicrobium Cellulans 191.

dc.creatorFleuri, Luciana F
dc.creatorKawaguti, Haroldo Y
dc.creatorSato, Hélia H
dc.date2009-Jul
dc.date2015-11-27T13:15:51Z
dc.date2015-11-27T13:15:51Z
dc.date.accessioned2018-03-29T01:09:58Z
dc.date.available2018-03-29T01:09:58Z
dc.identifierBrazilian Journal Of Microbiology : [publication Of The Brazilian Society For Microbiology]. v. 40, n. 3, p. 623-30, 2009-Jul.
dc.identifier1517-8382
dc.identifier10.1590/S1517-838220090003000026
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/24031407
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198616
dc.identifier24031407
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298849
dc.descriptionThis study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25°C and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25°C and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts.
dc.description40
dc.description623-30
dc.languageeng
dc.relationBrazilian Journal Of Microbiology : [publication Of The Brazilian Society For Microbiology]
dc.relationBraz. J. Microbiol.
dc.rightsaberto
dc.rights
dc.sourcePubMed
dc.subjectCellulosimicrobium Cellulans
dc.subjectChitinase
dc.subjectFungal Lysis
dc.subjectProtoplasts
dc.titleProduction, Purification And Application Of Extracellular Chitinase From Cellulosimicrobium Cellulans 191.
dc.typeArtículos de revistas


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