Adsorption Of Human Serum Proteins Onto Tren-agarose: Purification Of Human Igg By Negative Chromatography.

dc.creatorBresolin, Igor Tadeu Lazzarotto
dc.creatorBorsoi-Ribeiro, Mariana
dc.creatorCaro, Juliana Rodrigues
dc.creatordos Santos, Francine Petit
dc.creatorde Castro, Marina Polesi
dc.creatorBueno, Sonia Maria Alves
dc.date2009-Jan
dc.date2015-11-27T13:15:47Z
dc.date2015-11-27T13:15:47Z
dc.date.accessioned2018-03-29T01:09:50Z
dc.date.available2018-03-29T01:09:50Z
dc.identifierJournal Of Chromatography. B, Analytical Technologies In The Biomedical And Life Sciences. v. 877, n. 1-2, p. 17-23, 2009-Jan.
dc.identifier1570-0232
dc.identifier10.1016/j.jchromb.2008.11.008
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19036647
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198578
dc.identifier19036647
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298811
dc.descriptionTris(2-aminoethyl)amine (TREN) - a chelating agent used in IMAC - immobilized onto agarose gel was evaluated for the purification of IgG from human serum by negative chromatography. A one-step purification process allowed the recovery of 73.3% of the loaded IgG in the nonretained fractions with purity of 90-95% (based on total protein concentration and nephelometric analysis of albumin, transferrin, and immunoglobulins A, G, and M). The binding capacity was relatively high (66.63 mg of human serum protein/mL). These results suggest that this negative chromatography is a potential technique for purification of IgG from human serum.
dc.description877
dc.description17-23
dc.languageeng
dc.relationJournal Of Chromatography. B, Analytical Technologies In The Biomedical And Life Sciences
dc.relationJ. Chromatogr. B Analyt. Technol. Biomed. Life Sci.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdsorption
dc.subjectBlood Proteins
dc.subjectBuffers
dc.subjectChromatography, Liquid
dc.subjectElectrophoresis, Polyacrylamide Gel
dc.subjectHumans
dc.subjectImmunoglobulin G
dc.subjectSensitivity And Specificity
dc.subjectSepharose
dc.titleAdsorption Of Human Serum Proteins Onto Tren-agarose: Purification Of Human Igg By Negative Chromatography.
dc.typeArtículos de revistas


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