Nanocytotoxicity: Violacein And Violacein-loaded Poly (d, L-lactide-co-glycolide) Nanoparticles Acting On Human Leukemic Cells.

dc.creatorMelo, Patricia S
dc.creatorDe Azevedo, Marcelo M M
dc.creatorFrungillo, Lucas
dc.creatorAnazetti, Maristela C
dc.creatorMarcato, Priscyla D
dc.creatorDuran, Nelson
dc.date2009-Apr
dc.date2015-11-27T13:15:44Z
dc.date2015-11-27T13:15:44Z
dc.date.accessioned2018-03-29T01:09:46Z
dc.date.available2018-03-29T01:09:46Z
dc.identifierJournal Of Biomedical Nanotechnology. v. 5, n. 2, p. 192-201, 2009-Apr.
dc.identifier1550-7033
dc.identifier
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/20055097
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198561
dc.identifier20055097
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298794
dc.descriptionViolacein is a compound obtained from Chromobacterium violaceum, a bacterium found in the Amazonian region. Violacein-loaded poly (D, L-lactide-co-glycolide) nanoparticles has a similar inhibitory effect evaluated by trypan blue assay on leukemic HL60 cells than the free form. However, the cytotoxic effects evaluated by phosphatase activity and MTT reduction assays were lower for the encapsulated form than for free violacein. Based on morphological changes, violacein and violacein entrapped in nanoparticles were found to induce terminal differentiation (assessed by nitro blue tetrazolium reduction) in HL60 cells. Thus, both formulations inhibit HL60 cell growth in vitro, partly by inducing cytotoxic effects and cell differentiation. Flow cytometric analysis of HL60 cells after treatment for 12 h showed that violacein-loaded PLGA induced apoptosis, with maximum cell death at a concentration of 2 microM. Violacein and violacein/PLGA induced opposite effects on the mitochondrial swelling which indicates altered mitochondrial function. The mitochondrial activity was also checked by flow cytometry studies. Labelled cells with the probe JC1 displayed a basal hypopolarized status of the mitochondria in treated cells. Based on morphological changes, alterations in phospholipid asymmetry and changes in mitochondrial polarization, violacein and nanoparticles containing violacein were found to trigger cell death by apoptosis. These methodologies are promising as diagnostic and mechanistic effects of nanoparticles in cell cultures.
dc.description5
dc.description192-201
dc.languageeng
dc.relationJournal Of Biomedical Nanotechnology
dc.relationJ Biomed Nanotechnol
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectApoptosis
dc.subjectCell Survival
dc.subjectCrystallization
dc.subjectDrug Carriers
dc.subjectHl-60 Cells
dc.subjectHumans
dc.subjectIndoles
dc.subjectLactic Acid
dc.subjectMacromolecular Substances
dc.subjectMaterials Testing
dc.subjectMolecular Conformation
dc.subjectNanostructures
dc.subjectParticle Size
dc.subjectPolyglycolic Acid
dc.subjectSurface Properties
dc.titleNanocytotoxicity: Violacein And Violacein-loaded Poly (d, L-lactide-co-glycolide) Nanoparticles Acting On Human Leukemic Cells.
dc.typeArtículos de revistas


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