Dibucaine Effects On Structural And Elastic Properties Of Lipid Bilayers.

dc.creatorLorite, G S
dc.creatorNobre, T M
dc.creatorZaniquelli, M E D
dc.creatorde Paula, E
dc.creatorCotta, M A
dc.date2009-Feb
dc.date2015-11-27T13:15:36Z
dc.date2015-11-27T13:15:36Z
dc.date.accessioned2018-03-29T01:09:32Z
dc.date.available2018-03-29T01:09:32Z
dc.identifierBiophysical Chemistry. v. 139, n. 2-3, p. 75-83, 2009-Feb.
dc.identifier1873-4200
dc.identifier10.1016/j.bpc.2008.10.006
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19010585
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198501
dc.identifier19010585
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298734
dc.descriptionIn this work we report the interaction effects of the local anesthetic dibucaine (DBC) with lipid patches in model membranes by Atomic Force Microscopy (AFM). Supported lipid bilayers (egg phosphatidylcholine, EPC and dimyristoylphosphatidylcholine, DMPC) were prepared by fusion of unilamellar vesicles on mica and imaged in aqueous media. The AFM images show irregularly distributed and sized EPC patches on mica. On the other hand DMPC formation presents extensive bilayer regions on top of which multibilayer patches are formed. In the presence of DBC we observed a progressive disruption of these patches, but for DMPC bilayers this process occurred more slowly than for EPC. In both cases, phase images show the formation of small structures on the bilayer surface suggesting an effect on the elastic properties of the bilayers when DBC is present. Dynamic surface tension and dilatational surface elasticity measurements of EPC and DMPC monolayers in the presence of DBC by the pendant drop technique were also performed, in order to elucidate these results. The curve of lipid monolayer elasticity versus DBC concentration, for both EPC and DMPC cases, shows a maximum for the surface elasticity modulus at the same concentration where we observed the disruption of the bilayer by AFM. Our results suggest that changes in the local curvature of the bilayer induced by DBC could explain the anesthetic action in membranes.
dc.description139
dc.description75-83
dc.languageeng
dc.relationBiophysical Chemistry
dc.relationBiophys. Chem.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdsorption
dc.subjectAluminum Silicates
dc.subjectAnesthetics, Local
dc.subjectDibucaine
dc.subjectDimyristoylphosphatidylcholine
dc.subjectElasticity
dc.subjectKinetics
dc.subjectLipid Bilayers
dc.subjectMicroscopy, Atomic Force
dc.subjectPhosphatidylcholines
dc.subjectSurface Properties
dc.subjectTime Factors
dc.titleDibucaine Effects On Structural And Elastic Properties Of Lipid Bilayers.
dc.typeArtículos de revistas


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