Dna Methylation Status Of The Il8 Gene Promoter In Oral Cells Of Smokers And Non-smokers With Chronic Periodontitis.

dc.creatorOliveira, Naila F P
dc.creatorDamm, Gilcy R
dc.creatorAndia, Denise C
dc.creatorSalmon, Cristiane
dc.creatorNociti, Francisco H
dc.creatorLine, Sérgio R P
dc.creatorde Souza, Ana Paula
dc.date2009-Sep
dc.date2015-11-27T13:15:28Z
dc.date2015-11-27T13:15:28Z
dc.date.accessioned2018-03-29T01:09:16Z
dc.date.available2018-03-29T01:09:16Z
dc.identifierJournal Of Clinical Periodontology. v. 36, n. 9, p. 719-25, 2009-Sep.
dc.identifier1600-051X
dc.identifier10.1111/j.1600-051X.2009.01446.x
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19659670
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198430
dc.identifier19659670
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298663
dc.descriptionThis study analysed the status of DNA methylation in the promoter region of the IL8 gene in oral mucosa cells from healthy, smoker and non-smoker subjects with chronic periodontitis and compared these findings among groups with mRNA levels. Genomic DNA from epithelial oral cells of 41 healthy subjects, 30 smokers with chronic periodontitis and 40 non-smokers with chronic periodontitis were purified and modified by sodium bisulphite. Genomic DNA from blood leucocytes and gingival cells from biopsies of 13 subjects of each group were also purified and modified by sodium bisulphite. Modified DNA was submitted by methylation-specific polymerase chain reaction (PCR) (MSP), electrophoresed on 10% polyacrylamide gels and stained with SYBR Gold. Total RNA from gingival cells was also isolated using the TRIzol reagent, and real-time PCR performance was used to detect the levels of interleukin-8 mRNA. Our results indicate that individuals with chronic periodontitis, independent of smoking habit, have a higher percentage of hipomethylation of the IL8 gene than those controls in epithelial oral cells (p<0.0001), and expression of higher levels of interleukin-8 (IL-8) mRNA than controls in gingival cells (p=0.007). No significant differences among groups were observed in gingival cells and blood cells. We conclude that inflammation in the oral mucosa might lead to changes in the DNA methylation status of the IL8 gene in epithelial oral cells.
dc.description36
dc.description719-25
dc.languageeng
dc.relationJournal Of Clinical Periodontology
dc.relationJ. Clin. Periodontol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectAdult
dc.subjectCase-control Studies
dc.subjectChronic Periodontitis
dc.subjectCpg Islands
dc.subjectDna Methylation
dc.subjectEpigenesis, Genetic
dc.subjectEpithelial Cells
dc.subjectFemale
dc.subjectHumans
dc.subjectInterleukin-8
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMouth Mucosa
dc.subjectPolymerase Chain Reaction
dc.subjectPromoter Regions, Genetic
dc.subjectSmoking
dc.titleDna Methylation Status Of The Il8 Gene Promoter In Oral Cells Of Smokers And Non-smokers With Chronic Periodontitis.
dc.typeArtículos de revistas


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