Pip4kiia And Beta-globin: Transcripts Differentially Expressed In Reticulocytes And Associated With High Levels Of Hb H In Two Siblings With Hb H Disease.

dc.creatorWenning, Marcia R S C
dc.creatorMello, Maricilda P
dc.creatorAndrade, Tiago G
dc.creatorLanaro, Carolina
dc.creatorAlbuquerque, Dulcineia M
dc.creatorSaad, Sara T O
dc.creatorCosta, Fernando F
dc.creatorSonati, Maria F
dc.date2009-Nov
dc.date2015-11-27T13:15:27Z
dc.date2015-11-27T13:15:27Z
dc.date.accessioned2018-03-29T01:09:16Z
dc.date.available2018-03-29T01:09:16Z
dc.identifierEuropean Journal Of Haematology. v. 83, n. 5, p. 490-3, 2009-Nov.
dc.identifier1600-0609
dc.identifier10.1111/j.1600-0609.2009.01325.x
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/19656170
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198429
dc.identifier19656170
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1298662
dc.descriptionWe are reporting here the results of differential gene expression experiments comparing two siblings, a 21-yr-old male and a 19-yr-old female, with the same alpha-thalassemia genotype (-alpha(3.7)/(--SEA)) and quite different levels of Hb H in the peripheral blood (18.7 and 5%, respectively). By using mRNA differential-display reverse-transcription-PCR and suppression subtractive hybridization, two main transcripts were selected in both procedures and validated by qRT-PCR, one corresponding to the phosphatidylinositol phosphate 4-kinase type II-alpha (PIP4KIIA) gene and the other to the beta-globin gene, both over expressed in the patient with the higher percentage of Hb H. Type II PIP kinases produce phosphatidylinositol 4,5 biphosphate, a critical and pleiotropic regulatory molecule involved in diverse cellular activities, including gene expression. Our results suggest that PIP4KIIA may be one of the factors related to the regulation of the beta-globin gene expression and the different levels of Hb H in alpha-thalassemic patients.
dc.description83
dc.description490-3
dc.languageeng
dc.relationEuropean Journal Of Haematology
dc.relationEur. J. Haematol.
dc.rightsfechado
dc.rights
dc.sourcePubMed
dc.subjectFemale
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectHemoglobin H
dc.subjectHumans
dc.subjectMale
dc.subjectPhosphatidylinositol 4,5-diphosphate
dc.subjectPhosphotransferases (alcohol Group Acceptor)
dc.subjectReticulocytes
dc.subjectSiblings
dc.subjectYoung Adult
dc.subjectAlpha-thalassemia
dc.subjectBeta-globins
dc.titlePip4kiia And Beta-globin: Transcripts Differentially Expressed In Reticulocytes And Associated With High Levels Of Hb H In Two Siblings With Hb H Disease.
dc.typeArtículos de revistas


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